Multiple-channel scaffolds to promote spinal cord axon regeneration

Michael J. Moore, Jonathan A. Friedman, Eric B. Lewellyn, Sara M. Mantila, Aaron Krych, Syed Ameenuddin, Andrew M. Knight, Lichun Lu, Bradford L. Currier, Robert J. Spinner, Richard W. Marsh, Anthony John Windebank, Michael J Yaszemski

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

As molecular, cellular, and tissue-level treatments for spinal cord injury are discovered, it is likely that combinations of such treatments will be necessary to elicit functional recovery in animal models or patients. We describe multiple-channel, biodegradable scaffolds that serve as the basis for a model to investigate simultaneously the effects on axon regeneration of scaffold architecture, transplanted cells, and locally delivered molecular agents. Poly(lactic-co-glycolic acid) (PLGA) with copolymer ratio 85:15 was used for these initial experiments. Injection molding with rapid solvent evaporation resulted in scaffolds with a plurality of distinct channels running parallel along the length of the scaffolds. The feasibility of creating scaffolds with various channel sizes and geometries was demonstrated. Walls separating open channels were found to possess void fractions as high as 89%, with accessible void fractions as high as 90% through connections 220 μm or larger. Scaffolds degraded in vitro over a period of 30 weeks, over which time-sustained delivery of a surrogate drug was observed for 12 weeks. Primary neonatal Schwann cells were distributed in the channels of the scaffold and remained viable in tissue culture for at least 48 h. Schwann-cell containing scaffolds implanted into transected adult rat spinal cords contained regenerating axons at one month post-operation. Axon regeneration was demonstrated by three-dimensional reconstruction of serial histological sections.

Original languageEnglish (US)
Pages (from-to)419-429
Number of pages11
JournalBiomaterials
Volume27
Issue number3
DOIs
StatePublished - Jan 2006

Fingerprint

Spinal Cord Regeneration
Scaffolds
Axons
Schwann Cells
Regeneration
Spinal Cord Injuries
Void fraction
Spinal Cord
Animal Models
Injections
Tissue culture
Therapeutics
Pharmaceutical Preparations
Scaffolds (biology)
Injection molding
Rats
Evaporation
Animals
Copolymers
Cells

Keywords

  • Drug release
  • Image analysis
  • Microstructure
  • Nerve tissue engineering
  • Scaffold
  • Schwann cell

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Multiple-channel scaffolds to promote spinal cord axon regeneration. / Moore, Michael J.; Friedman, Jonathan A.; Lewellyn, Eric B.; Mantila, Sara M.; Krych, Aaron; Ameenuddin, Syed; Knight, Andrew M.; Lu, Lichun; Currier, Bradford L.; Spinner, Robert J.; Marsh, Richard W.; Windebank, Anthony John; Yaszemski, Michael J.

In: Biomaterials, Vol. 27, No. 3, 01.2006, p. 419-429.

Research output: Contribution to journalArticle

Moore, MJ, Friedman, JA, Lewellyn, EB, Mantila, SM, Krych, A, Ameenuddin, S, Knight, AM, Lu, L, Currier, BL, Spinner, RJ, Marsh, RW, Windebank, AJ & Yaszemski, MJ 2006, 'Multiple-channel scaffolds to promote spinal cord axon regeneration', Biomaterials, vol. 27, no. 3, pp. 419-429. https://doi.org/10.1016/j.biomaterials.2005.07.045
Moore MJ, Friedman JA, Lewellyn EB, Mantila SM, Krych A, Ameenuddin S et al. Multiple-channel scaffolds to promote spinal cord axon regeneration. Biomaterials. 2006 Jan;27(3):419-429. https://doi.org/10.1016/j.biomaterials.2005.07.045
Moore, Michael J. ; Friedman, Jonathan A. ; Lewellyn, Eric B. ; Mantila, Sara M. ; Krych, Aaron ; Ameenuddin, Syed ; Knight, Andrew M. ; Lu, Lichun ; Currier, Bradford L. ; Spinner, Robert J. ; Marsh, Richard W. ; Windebank, Anthony John ; Yaszemski, Michael J. / Multiple-channel scaffolds to promote spinal cord axon regeneration. In: Biomaterials. 2006 ; Vol. 27, No. 3. pp. 419-429.
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