Multiple cellular phenotypes in an insertional mutation in mouse affecting bone development

N. L. Nadon, C. J. Doersen, D. A. Lade, K. Medina, L. Thompson, M. Rohrer, J. M. Gimble

Research output: Contribution to journalArticle

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Abstract

The 6093 line of transgenic mice exhibits altered bone development as a result of an insertional mutation by the transgene. Female transgenic mice show a marked kyphosis as early as 2 weeks of age. Vertebrae from female mice have lower total bone area and mineral content than age-matched, gender-matched controls, although the bone mineral density is not changed. The femur and tibia exhibit the opposite effect - increased bone area and mineral content. Fluorescent bone label experiments indicated an increased rate of bone mineral deposition in the femur during the early postnatal growth period, and bone marrow from femurs of 6093 females had increased numbers of fibroblast colony-forming units. Transgenic females also are obese and have altered thymocyte development, suggesting that the insertional mutation affects multiple cell populations. We hypothesize that these phenotypes arise as a result of an alteration in the function or developmental potential of a stromal cell or mesenchymal stem cell.

Original languageEnglish (US)
Pages (from-to)449-454
Number of pages6
JournalCalcified Tissue International
Volume67
Issue number6
DOIs
StatePublished - Dec 1 2000

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Keywords

  • Bone
  • Kyphosis
  • Mesenchymal stem cell
  • Stromal cell
  • Transgenic mice

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

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