Abstract
The translation of engineered tissues into clinic requires robust monitoring of tissue development, both in vitro and in vivo. Traditional methods for the same are destructive, inefficient in time and cost and do not allow time-lapse measurements from the same sample or animal. This study reports on the ability of time-resolved fluorescence and ultrasound measurements for non-destructive characterization of explanted tissue engineered vascular grafts. Results show that TRFS and FLIm are able to assess alterations in luminal composition namely elastin, collagen and cellular (hyperplasia) content via changes in fluorescence lifetime values between normal and grafted tissue. These observations are complemented by structural changes observed in UBM pertaining to graft integration and intimal thickness over the grafted region. These results encourage the future application of a catheter-based technique that combines these imaging modalities for non-destructive characterization of vascular grafts in vivo.
| Original language | English (US) |
|---|---|
| Title of host publication | Photonic Therapeutics and Diagnostics XI |
| Editors | Hyun Wook Kang, Justus F. Ilgner, Melissa C. Skala, Laura Marcu, Haishan Zeng, Mark W. Dewhirst, Kenton W. Gregory, Andreas Mandelis, Bernard Choi, Brian Jet-Fei Wong, Claus-Peter Richter, Guillermo J. Tearney, Alfred Nuttal, Nikiforos Kollias |
| Publisher | SPIE |
| Volume | 9303 |
| ISBN (Electronic) | 9781628413939 |
| DOIs | |
| State | Published - Jan 1 2015 |
| Externally published | Yes |
| Event | Photonic Therapeutics and Diagnostics XI - San Francisco, United States Duration: Feb 7 2015 → Feb 8 2015 |
Other
| Other | Photonic Therapeutics and Diagnostics XI |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 2/7/15 → 2/8/15 |
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Keywords
- Time-resolved fluorescence
- Tissue characterization
- Tissue engineering
- Ultrasound
- Vascular grafts
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Radiology Nuclear Medicine and imaging
Cite this
Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound. / Fatakdawala, Hussain; Griffiths, Leigh; Humphrey, Sterling; Marcu, Laura.
Photonic Therapeutics and Diagnostics XI. ed. / Hyun Wook Kang; Justus F. Ilgner; Melissa C. Skala; Laura Marcu; Haishan Zeng; Mark W. Dewhirst; Kenton W. Gregory; Andreas Mandelis; Bernard Choi; Brian Jet-Fei Wong; Claus-Peter Richter; Guillermo J. Tearney; Alfred Nuttal; Nikiforos Kollias. Vol. 9303 SPIE, 2015. 930335.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
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TY - GEN
T1 - Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound
AU - Fatakdawala, Hussain
AU - Griffiths, Leigh
AU - Humphrey, Sterling
AU - Marcu, Laura
PY - 2015/1/1
Y1 - 2015/1/1
N2 - The translation of engineered tissues into clinic requires robust monitoring of tissue development, both in vitro and in vivo. Traditional methods for the same are destructive, inefficient in time and cost and do not allow time-lapse measurements from the same sample or animal. This study reports on the ability of time-resolved fluorescence and ultrasound measurements for non-destructive characterization of explanted tissue engineered vascular grafts. Results show that TRFS and FLIm are able to assess alterations in luminal composition namely elastin, collagen and cellular (hyperplasia) content via changes in fluorescence lifetime values between normal and grafted tissue. These observations are complemented by structural changes observed in UBM pertaining to graft integration and intimal thickness over the grafted region. These results encourage the future application of a catheter-based technique that combines these imaging modalities for non-destructive characterization of vascular grafts in vivo.
AB - The translation of engineered tissues into clinic requires robust monitoring of tissue development, both in vitro and in vivo. Traditional methods for the same are destructive, inefficient in time and cost and do not allow time-lapse measurements from the same sample or animal. This study reports on the ability of time-resolved fluorescence and ultrasound measurements for non-destructive characterization of explanted tissue engineered vascular grafts. Results show that TRFS and FLIm are able to assess alterations in luminal composition namely elastin, collagen and cellular (hyperplasia) content via changes in fluorescence lifetime values between normal and grafted tissue. These observations are complemented by structural changes observed in UBM pertaining to graft integration and intimal thickness over the grafted region. These results encourage the future application of a catheter-based technique that combines these imaging modalities for non-destructive characterization of vascular grafts in vivo.
KW - Time-resolved fluorescence
KW - Tissue characterization
KW - Tissue engineering
KW - Ultrasound
KW - Vascular grafts
UR - http://www.scopus.com/inward/record.url?scp=84926617197&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84926617197&partnerID=8YFLogxK
U2 - 10.1117/12.2078278
DO - 10.1117/12.2078278
M3 - Conference contribution
VL - 9303
BT - Photonic Therapeutics and Diagnostics XI
A2 - Kang, Hyun Wook
A2 - Ilgner, Justus F.
A2 - Skala, Melissa C.
A2 - Marcu, Laura
A2 - Zeng, Haishan
A2 - Dewhirst, Mark W.
A2 - Gregory, Kenton W.
A2 - Mandelis, Andreas
A2 - Choi, Bernard
A2 - Wong, Brian Jet-Fei
A2 - Richter, Claus-Peter
A2 - Tearney, Guillermo J.
A2 - Nuttal, Alfred
A2 - Kollias, Nikiforos
PB - SPIE
ER -