Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound

Hussain Fatakdawala, Leigh Griffiths, Sterling Humphrey, Laura Marcu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The translation of engineered tissues into clinic requires robust monitoring of tissue development, both in vitro and in vivo. Traditional methods for the same are destructive, inefficient in time and cost and do not allow time-lapse measurements from the same sample or animal. This study reports on the ability of time-resolved fluorescence and ultrasound measurements for non-destructive characterization of explanted tissue engineered vascular grafts. Results show that TRFS and FLIm are able to assess alterations in luminal composition namely elastin, collagen and cellular (hyperplasia) content via changes in fluorescence lifetime values between normal and grafted tissue. These observations are complemented by structural changes observed in UBM pertaining to graft integration and intimal thickness over the grafted region. These results encourage the future application of a catheter-based technique that combines these imaging modalities for non-destructive characterization of vascular grafts in vivo.

Original languageEnglish (US)
Title of host publicationPhotonic Therapeutics and Diagnostics XI
EditorsHyun Wook Kang, Justus F. Ilgner, Melissa C. Skala, Laura Marcu, Haishan Zeng, Mark W. Dewhirst, Kenton W. Gregory, Andreas Mandelis, Bernard Choi, Brian Jet-Fei Wong, Claus-Peter Richter, Guillermo J. Tearney, Alfred Nuttal, Nikiforos Kollias
PublisherSPIE
Volume9303
ISBN (Electronic)9781628413939
DOIs
StatePublished - Jan 1 2015
Externally publishedYes
EventPhotonic Therapeutics and Diagnostics XI - San Francisco, United States
Duration: Feb 7 2015Feb 8 2015

Other

OtherPhotonic Therapeutics and Diagnostics XI
CountryUnited States
CitySan Francisco
Period2/7/152/8/15

Keywords

  • Time-resolved fluorescence
  • Tissue characterization
  • Tissue engineering
  • Ultrasound
  • Vascular grafts

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Fatakdawala, H., Griffiths, L., Humphrey, S., & Marcu, L. (2015). Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound. In H. W. Kang, J. F. Ilgner, M. C. Skala, L. Marcu, H. Zeng, M. W. Dewhirst, K. W. Gregory, A. Mandelis, B. Choi, B. J-F. Wong, C-P. Richter, G. J. Tearney, A. Nuttal, & N. Kollias (Eds.), Photonic Therapeutics and Diagnostics XI (Vol. 9303). [930335] SPIE. https://doi.org/10.1117/12.2078278