Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound

Hussain Fatakdawala, Leigh Griffiths, Sterling Humphrey, Laura Marcu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The translation of engineered tissues into clinic requires robust monitoring of tissue development, both in vitro and in vivo. Traditional methods for the same are destructive, inefficient in time and cost and do not allow time-lapse measurements from the same sample or animal. This study reports on the ability of time-resolved fluorescence and ultrasound measurements for non-destructive characterization of explanted tissue engineered vascular grafts. Results show that TRFS and FLIm are able to assess alterations in luminal composition namely elastin, collagen and cellular (hyperplasia) content via changes in fluorescence lifetime values between normal and grafted tissue. These observations are complemented by structural changes observed in UBM pertaining to graft integration and intimal thickness over the grafted region. These results encourage the future application of a catheter-based technique that combines these imaging modalities for non-destructive characterization of vascular grafts in vivo.

Original languageEnglish (US)
Title of host publicationPhotonic Therapeutics and Diagnostics XI
EditorsHyun Wook Kang, Justus F. Ilgner, Melissa C. Skala, Laura Marcu, Haishan Zeng, Mark W. Dewhirst, Kenton W. Gregory, Andreas Mandelis, Bernard Choi, Brian Jet-Fei Wong, Claus-Peter Richter, Guillermo J. Tearney, Alfred Nuttal, Nikiforos Kollias
PublisherSPIE
Volume9303
ISBN (Electronic)9781628413939
DOIs
StatePublished - Jan 1 2015
Externally publishedYes
EventPhotonic Therapeutics and Diagnostics XI - San Francisco, United States
Duration: Feb 7 2015Feb 8 2015

Other

OtherPhotonic Therapeutics and Diagnostics XI
CountryUnited States
CitySan Francisco
Period2/7/152/8/15

Fingerprint

Multimodal Imaging
Grafts
Blood Vessels
Fluorescence
Ultrasonics
Tissue
Transplants
Imaging techniques
fluorescence
Tunica Intima
Blood Vessel Prosthesis
Elastin
elastin
Hyperplasia
Catheters
Reference Values
Collagen
collagens
animals
Costs and Cost Analysis

Keywords

  • Time-resolved fluorescence
  • Tissue characterization
  • Tissue engineering
  • Ultrasound
  • Vascular grafts

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Fatakdawala, H., Griffiths, L., Humphrey, S., & Marcu, L. (2015). Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound. In H. W. Kang, J. F. Ilgner, M. C. Skala, L. Marcu, H. Zeng, M. W. Dewhirst, K. W. Gregory, A. Mandelis, B. Choi, B. J-F. Wong, C-P. Richter, G. J. Tearney, A. Nuttal, ... N. Kollias (Eds.), Photonic Therapeutics and Diagnostics XI (Vol. 9303). [930335] SPIE. https://doi.org/10.1117/12.2078278

Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound. / Fatakdawala, Hussain; Griffiths, Leigh; Humphrey, Sterling; Marcu, Laura.

Photonic Therapeutics and Diagnostics XI. ed. / Hyun Wook Kang; Justus F. Ilgner; Melissa C. Skala; Laura Marcu; Haishan Zeng; Mark W. Dewhirst; Kenton W. Gregory; Andreas Mandelis; Bernard Choi; Brian Jet-Fei Wong; Claus-Peter Richter; Guillermo J. Tearney; Alfred Nuttal; Nikiforos Kollias. Vol. 9303 SPIE, 2015. 930335.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fatakdawala, H, Griffiths, L, Humphrey, S & Marcu, L 2015, Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound. in HW Kang, JF Ilgner, MC Skala, L Marcu, H Zeng, MW Dewhirst, KW Gregory, A Mandelis, B Choi, BJ-F Wong, C-P Richter, GJ Tearney, A Nuttal & N Kollias (eds), Photonic Therapeutics and Diagnostics XI. vol. 9303, 930335, SPIE, Photonic Therapeutics and Diagnostics XI, San Francisco, United States, 2/7/15. https://doi.org/10.1117/12.2078278
Fatakdawala H, Griffiths L, Humphrey S, Marcu L. Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound. In Kang HW, Ilgner JF, Skala MC, Marcu L, Zeng H, Dewhirst MW, Gregory KW, Mandelis A, Choi B, Wong BJ-F, Richter C-P, Tearney GJ, Nuttal A, Kollias N, editors, Photonic Therapeutics and Diagnostics XI. Vol. 9303. SPIE. 2015. 930335 https://doi.org/10.1117/12.2078278
Fatakdawala, Hussain ; Griffiths, Leigh ; Humphrey, Sterling ; Marcu, Laura. / Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound. Photonic Therapeutics and Diagnostics XI. editor / Hyun Wook Kang ; Justus F. Ilgner ; Melissa C. Skala ; Laura Marcu ; Haishan Zeng ; Mark W. Dewhirst ; Kenton W. Gregory ; Andreas Mandelis ; Bernard Choi ; Brian Jet-Fei Wong ; Claus-Peter Richter ; Guillermo J. Tearney ; Alfred Nuttal ; Nikiforos Kollias. Vol. 9303 SPIE, 2015.
@inproceedings{bf50981fa04f4c78923f0a0d3e1aa999,
title = "Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound",
abstract = "The translation of engineered tissues into clinic requires robust monitoring of tissue development, both in vitro and in vivo. Traditional methods for the same are destructive, inefficient in time and cost and do not allow time-lapse measurements from the same sample or animal. This study reports on the ability of time-resolved fluorescence and ultrasound measurements for non-destructive characterization of explanted tissue engineered vascular grafts. Results show that TRFS and FLIm are able to assess alterations in luminal composition namely elastin, collagen and cellular (hyperplasia) content via changes in fluorescence lifetime values between normal and grafted tissue. These observations are complemented by structural changes observed in UBM pertaining to graft integration and intimal thickness over the grafted region. These results encourage the future application of a catheter-based technique that combines these imaging modalities for non-destructive characterization of vascular grafts in vivo.",
keywords = "Time-resolved fluorescence, Tissue characterization, Tissue engineering, Ultrasound, Vascular grafts",
author = "Hussain Fatakdawala and Leigh Griffiths and Sterling Humphrey and Laura Marcu",
year = "2015",
month = "1",
day = "1",
doi = "10.1117/12.2078278",
language = "English (US)",
volume = "9303",
editor = "Kang, {Hyun Wook} and Ilgner, {Justus F.} and Skala, {Melissa C.} and Laura Marcu and Haishan Zeng and Dewhirst, {Mark W.} and Gregory, {Kenton W.} and Andreas Mandelis and Bernard Choi and Wong, {Brian Jet-Fei} and Claus-Peter Richter and Tearney, {Guillermo J.} and Alfred Nuttal and Nikiforos Kollias",
booktitle = "Photonic Therapeutics and Diagnostics XI",
publisher = "SPIE",

}

TY - GEN

T1 - Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound

AU - Fatakdawala, Hussain

AU - Griffiths, Leigh

AU - Humphrey, Sterling

AU - Marcu, Laura

PY - 2015/1/1

Y1 - 2015/1/1

N2 - The translation of engineered tissues into clinic requires robust monitoring of tissue development, both in vitro and in vivo. Traditional methods for the same are destructive, inefficient in time and cost and do not allow time-lapse measurements from the same sample or animal. This study reports on the ability of time-resolved fluorescence and ultrasound measurements for non-destructive characterization of explanted tissue engineered vascular grafts. Results show that TRFS and FLIm are able to assess alterations in luminal composition namely elastin, collagen and cellular (hyperplasia) content via changes in fluorescence lifetime values between normal and grafted tissue. These observations are complemented by structural changes observed in UBM pertaining to graft integration and intimal thickness over the grafted region. These results encourage the future application of a catheter-based technique that combines these imaging modalities for non-destructive characterization of vascular grafts in vivo.

AB - The translation of engineered tissues into clinic requires robust monitoring of tissue development, both in vitro and in vivo. Traditional methods for the same are destructive, inefficient in time and cost and do not allow time-lapse measurements from the same sample or animal. This study reports on the ability of time-resolved fluorescence and ultrasound measurements for non-destructive characterization of explanted tissue engineered vascular grafts. Results show that TRFS and FLIm are able to assess alterations in luminal composition namely elastin, collagen and cellular (hyperplasia) content via changes in fluorescence lifetime values between normal and grafted tissue. These observations are complemented by structural changes observed in UBM pertaining to graft integration and intimal thickness over the grafted region. These results encourage the future application of a catheter-based technique that combines these imaging modalities for non-destructive characterization of vascular grafts in vivo.

KW - Time-resolved fluorescence

KW - Tissue characterization

KW - Tissue engineering

KW - Ultrasound

KW - Vascular grafts

UR - http://www.scopus.com/inward/record.url?scp=84926617197&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84926617197&partnerID=8YFLogxK

U2 - 10.1117/12.2078278

DO - 10.1117/12.2078278

M3 - Conference contribution

AN - SCOPUS:84926617197

VL - 9303

BT - Photonic Therapeutics and Diagnostics XI

A2 - Kang, Hyun Wook

A2 - Ilgner, Justus F.

A2 - Skala, Melissa C.

A2 - Marcu, Laura

A2 - Zeng, Haishan

A2 - Dewhirst, Mark W.

A2 - Gregory, Kenton W.

A2 - Mandelis, Andreas

A2 - Choi, Bernard

A2 - Wong, Brian Jet-Fei

A2 - Richter, Claus-Peter

A2 - Tearney, Guillermo J.

A2 - Nuttal, Alfred

A2 - Kollias, Nikiforos

PB - SPIE

ER -