Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound

Hussain Fatakdawala; Leigh Griffiths; Sterling Humphrey; Laura Marcu

doi:10.1117/12.2078278

Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound

Hussain Fatakdawala, Leigh Griffiths, Sterling Humphrey, Laura Marcu

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The translation of engineered tissues into clinic requires robust monitoring of tissue development, both in vitro and in vivo. Traditional methods for the same are destructive, inefficient in time and cost and do not allow time-lapse measurements from the same sample or animal. This study reports on the ability of time-resolved fluorescence and ultrasound measurements for non-destructive characterization of explanted tissue engineered vascular grafts. Results show that TRFS and FLIm are able to assess alterations in luminal composition namely elastin, collagen and cellular (hyperplasia) content via changes in fluorescence lifetime values between normal and grafted tissue. These observations are complemented by structural changes observed in UBM pertaining to graft integration and intimal thickness over the grafted region. These results encourage the future application of a catheter-based technique that combines these imaging modalities for non-destructive characterization of vascular grafts in vivo.

Original language	English (US)
Title of host publication	Photonic Therapeutics and Diagnostics XI
Editors	Hyun Wook Kang, Justus F. Ilgner, Melissa C. Skala, Laura Marcu, Haishan Zeng, Mark W. Dewhirst, Kenton W. Gregory, Andreas Mandelis, Bernard Choi, Brian Jet-Fei Wong, Claus-Peter Richter, Guillermo J. Tearney, Alfred Nuttal, Nikiforos Kollias
Publisher	SPIE
Volume	9303
ISBN (Electronic)	9781628413939
DOIs	https://doi.org/10.1117/12.2078278
State	Published - Jan 1 2015
Externally published	Yes
Event	Photonic Therapeutics and Diagnostics XI - San Francisco, United States Duration: Feb 7 2015 → Feb 8 2015

Other

Other	Photonic Therapeutics and Diagnostics XI
Country/Territory	United States
City	San Francisco
Period	2/7/15 → 2/8/15

Keywords

Time-resolved fluorescence
Tissue characterization
Tissue engineering
Ultrasound
Vascular grafts

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2078278

Cite this

Fatakdawala, H., Griffiths, L., Humphrey, S., & Marcu, L. (2015). Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound. In H. W. Kang, J. F. Ilgner, M. C. Skala, L. Marcu, H. Zeng, M. W. Dewhirst, K. W. Gregory, A. Mandelis, B. Choi, B. J-F. Wong, C-P. Richter, G. J. Tearney, A. Nuttal, & N. Kollias (Eds.), Photonic Therapeutics and Diagnostics XI (Vol. 9303). [930335] SPIE. https://doi.org/10.1117/12.2078278

Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound. / Fatakdawala, Hussain; Griffiths, Leigh; Humphrey, Sterling; Marcu, Laura.

Photonic Therapeutics and Diagnostics XI. ed. / Hyun Wook Kang; Justus F. Ilgner; Melissa C. Skala; Laura Marcu; Haishan Zeng; Mark W. Dewhirst; Kenton W. Gregory; Andreas Mandelis; Bernard Choi; Brian Jet-Fei Wong; Claus-Peter Richter; Guillermo J. Tearney; Alfred Nuttal; Nikiforos Kollias. Vol. 9303 SPIE, 2015. 930335.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Fatakdawala, H, Griffiths, L, Humphrey, S & Marcu, L 2015, Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound. in HW Kang, JF Ilgner, MC Skala, L Marcu, H Zeng, MW Dewhirst, KW Gregory, A Mandelis, B Choi, BJ-F Wong, C-P Richter, GJ Tearney, A Nuttal & N Kollias (eds), Photonic Therapeutics and Diagnostics XI. vol. 9303, 930335, SPIE, Photonic Therapeutics and Diagnostics XI, San Francisco, United States, 2/7/15. https://doi.org/10.1117/12.2078278

Fatakdawala H, Griffiths L, Humphrey S, Marcu L. Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound. In Kang HW, Ilgner JF, Skala MC, Marcu L, Zeng H, Dewhirst MW, Gregory KW, Mandelis A, Choi B, Wong BJ-F, Richter C-P, Tearney GJ, Nuttal A, Kollias N, editors, Photonic Therapeutics and Diagnostics XI. Vol. 9303. SPIE. 2015. 930335 https://doi.org/10.1117/12.2078278

Fatakdawala, Hussain ; Griffiths, Leigh ; Humphrey, Sterling ; Marcu, Laura. / Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound. Photonic Therapeutics and Diagnostics XI. editor / Hyun Wook Kang ; Justus F. Ilgner ; Melissa C. Skala ; Laura Marcu ; Haishan Zeng ; Mark W. Dewhirst ; Kenton W. Gregory ; Andreas Mandelis ; Bernard Choi ; Brian Jet-Fei Wong ; Claus-Peter Richter ; Guillermo J. Tearney ; Alfred Nuttal ; Nikiforos Kollias. Vol. 9303 SPIE, 2015.

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abstract = "The translation of engineered tissues into clinic requires robust monitoring of tissue development, both in vitro and in vivo. Traditional methods for the same are destructive, inefficient in time and cost and do not allow time-lapse measurements from the same sample or animal. This study reports on the ability of time-resolved fluorescence and ultrasound measurements for non-destructive characterization of explanted tissue engineered vascular grafts. Results show that TRFS and FLIm are able to assess alterations in luminal composition namely elastin, collagen and cellular (hyperplasia) content via changes in fluorescence lifetime values between normal and grafted tissue. These observations are complemented by structural changes observed in UBM pertaining to graft integration and intimal thickness over the grafted region. These results encourage the future application of a catheter-based technique that combines these imaging modalities for non-destructive characterization of vascular grafts in vivo.",

keywords = "Time-resolved fluorescence, Tissue characterization, Tissue engineering, Ultrasound, Vascular grafts",

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AU - Fatakdawala, Hussain

AU - Griffiths, Leigh

AU - Humphrey, Sterling

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Y1 - 2015/1/1

N2 - The translation of engineered tissues into clinic requires robust monitoring of tissue development, both in vitro and in vivo. Traditional methods for the same are destructive, inefficient in time and cost and do not allow time-lapse measurements from the same sample or animal. This study reports on the ability of time-resolved fluorescence and ultrasound measurements for non-destructive characterization of explanted tissue engineered vascular grafts. Results show that TRFS and FLIm are able to assess alterations in luminal composition namely elastin, collagen and cellular (hyperplasia) content via changes in fluorescence lifetime values between normal and grafted tissue. These observations are complemented by structural changes observed in UBM pertaining to graft integration and intimal thickness over the grafted region. These results encourage the future application of a catheter-based technique that combines these imaging modalities for non-destructive characterization of vascular grafts in vivo.

AB - The translation of engineered tissues into clinic requires robust monitoring of tissue development, both in vitro and in vivo. Traditional methods for the same are destructive, inefficient in time and cost and do not allow time-lapse measurements from the same sample or animal. This study reports on the ability of time-resolved fluorescence and ultrasound measurements for non-destructive characterization of explanted tissue engineered vascular grafts. Results show that TRFS and FLIm are able to assess alterations in luminal composition namely elastin, collagen and cellular (hyperplasia) content via changes in fluorescence lifetime values between normal and grafted tissue. These observations are complemented by structural changes observed in UBM pertaining to graft integration and intimal thickness over the grafted region. These results encourage the future application of a catheter-based technique that combines these imaging modalities for non-destructive characterization of vascular grafts in vivo.

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ER -

Multimodal imaging of vascular grafts using time-resolved fluorescence and ultrasound

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Keywords

ASJC Scopus subject areas

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