Multihormonal regulation of ornithine decarboxylase activity in highly differentiated porcine granulosa cells in vitro

Johannes D Veldhuis, S. Sweinberg

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purified luteinizing hormone, but not follicle-stimulating hormone, elicited time- and dose-dependent stimulation of the cystolic enzyme, ornithine decarboxylase, in highly differentiated, porcine granulosa cells maintained in vitro in chemically defined medium. Enzymic induction was susceptible to inhibitors of protein and RNA synthesis, and was suppressed by selective direct and indirect inhibitors of ornithine decarboxylase. Physiologic concentrations of prostaglandin E 2 and L-epinephrine also enhanced enzymatic activity in a dose-dependent and saturable manner. Systematic comparison of the hormonal induction of ornithine decarboxylase in highly differentiated versus poorly differentiated granulosa cells revealed distinctive patterns of enzymic responsivity in relation to the degree of cytodifferentiation attained in vivo. This in vivo model is likely to permit further detailed examination of the molecular mechanisms subserving the hormonal control of ovarian ornithine decarboxylase activity in spontaneously differentiated granulosa cells maintained under chemically defined conditions in vitro.

Original languageEnglish (US)
Pages (from-to)213-220
Number of pages8
JournalJournal of Cellular Physiology
Volume108
Issue number2
StatePublished - 1981
Externally publishedYes

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Ornithine Decarboxylase
Granulosa Cells
Swine
Nucleic Acid Synthesis Inhibitors
Protein Synthesis Inhibitors
Follicle Stimulating Hormone
Luteinizing Hormone
Prostaglandins E
Epinephrine
Enzymes
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Multihormonal regulation of ornithine decarboxylase activity in highly differentiated porcine granulosa cells in vitro. / Veldhuis, Johannes D; Sweinberg, S.

In: Journal of Cellular Physiology, Vol. 108, No. 2, 1981, p. 213-220.

Research output: Contribution to journalArticle

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