Abstract
Purified luteinizing hormone, but not follicle-stimulating hormone, elicited time- and dose-dependent stimulation of the cystolic enzyme, ornithine decarboxylase, in highly differentiated, porcine granulosa cells maintained in vitro in chemically defined medium. Enzymic induction was susceptible to inhibitors of protein and RNA synthesis, and was suppressed by selective direct and indirect inhibitors of ornithine decarboxylase. Physiologic concentrations of prostaglandin E 2 and L-epinephrine also enhanced enzymatic activity in a dose-dependent and saturable manner. Systematic comparison of the hormonal induction of ornithine decarboxylase in highly differentiated versus poorly differentiated granulosa cells revealed distinctive patterns of enzymic responsivity in relation to the degree of cytodifferentiation attained in vivo. This in vivo model is likely to permit further detailed examination of the molecular mechanisms subserving the hormonal control of ovarian ornithine decarboxylase activity in spontaneously differentiated granulosa cells maintained under chemically defined conditions in vitro.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 213-220 |
| Number of pages | 8 |
| Journal | Journal of Cellular Physiology |
| Volume | 108 |
| Issue number | 2 |
| State | Published - 1981 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
- Physiology
Cite this
Multihormonal regulation of ornithine decarboxylase activity in highly differentiated porcine granulosa cells in vitro. / Veldhuis, Johannes D; Sweinberg, S.
In: Journal of Cellular Physiology, Vol. 108, No. 2, 1981, p. 213-220.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multihormonal regulation of ornithine decarboxylase activity in highly differentiated porcine granulosa cells in vitro
AU - Veldhuis, Johannes D
AU - Sweinberg, S.
PY - 1981
Y1 - 1981
N2 - Purified luteinizing hormone, but not follicle-stimulating hormone, elicited time- and dose-dependent stimulation of the cystolic enzyme, ornithine decarboxylase, in highly differentiated, porcine granulosa cells maintained in vitro in chemically defined medium. Enzymic induction was susceptible to inhibitors of protein and RNA synthesis, and was suppressed by selective direct and indirect inhibitors of ornithine decarboxylase. Physiologic concentrations of prostaglandin E 2 and L-epinephrine also enhanced enzymatic activity in a dose-dependent and saturable manner. Systematic comparison of the hormonal induction of ornithine decarboxylase in highly differentiated versus poorly differentiated granulosa cells revealed distinctive patterns of enzymic responsivity in relation to the degree of cytodifferentiation attained in vivo. This in vivo model is likely to permit further detailed examination of the molecular mechanisms subserving the hormonal control of ovarian ornithine decarboxylase activity in spontaneously differentiated granulosa cells maintained under chemically defined conditions in vitro.
AB - Purified luteinizing hormone, but not follicle-stimulating hormone, elicited time- and dose-dependent stimulation of the cystolic enzyme, ornithine decarboxylase, in highly differentiated, porcine granulosa cells maintained in vitro in chemically defined medium. Enzymic induction was susceptible to inhibitors of protein and RNA synthesis, and was suppressed by selective direct and indirect inhibitors of ornithine decarboxylase. Physiologic concentrations of prostaglandin E 2 and L-epinephrine also enhanced enzymatic activity in a dose-dependent and saturable manner. Systematic comparison of the hormonal induction of ornithine decarboxylase in highly differentiated versus poorly differentiated granulosa cells revealed distinctive patterns of enzymic responsivity in relation to the degree of cytodifferentiation attained in vivo. This in vivo model is likely to permit further detailed examination of the molecular mechanisms subserving the hormonal control of ovarian ornithine decarboxylase activity in spontaneously differentiated granulosa cells maintained under chemically defined conditions in vitro.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019447904&partnerID=8YFLogxK
M3 - Article
C2 - 6267083
AN - SCOPUS:0019447904
VL - 108
SP - 213
EP - 220
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
IS - 2
ER -