TY - JOUR
T1 - Multiexponential T2 and magnetization transfer MRI of demyelination and remyelination in murine spinal cord
AU - McCreary, Cheryl R.
AU - Bjarnason, Thorarin A.
AU - Skihar, Viktor
AU - Mitchell, J. Ross
AU - Yong, V. Wee
AU - Dunn, Jeff F.
N1 - Funding Information:
This work was funded by the MS Society of Canada, the Alberta Heritage Foundation for Medical Research, Informatics Circle of Research Excellence, the Canadian Institutes of Health Research, the National Institutes of Health (NIH RO1 EB002085), and the Canadian Foundation for Innovation.
PY - 2009/5/1
Y1 - 2009/5/1
N2 - Identification of remyelination is important in the evaluation of potential treatments of demyelinating diseases such as multiple sclerosis. Local injection of lysolecithin into the brain or spinal cord provides a murine model of demyelination with spontaneous remyelination. The aim of this study was to determine if quantitative, multicomponent T2 (qT2) analysis and magnetization transfer ratio (MTR), both indicative of myelin content, could detect changes in myelination, particularly remyelination, of the cervical spinal cord in mice treated with lysolecithin. We found that the myelin water fraction and geometric mean T2 value of the intra/extracellular water significantly decreased at 14 days then returned to control levels by 28 days after injury, corresponding to clearance of myelin debris and remyelination which was shown by eriochrome cyanine and oil red O staining of histological sections. The MTR was significantly decreased 14 days after lysolecithin injection, and remained low over the time course studied. Evidence of demyelination shown by both qT2 and MTR lagged behind the histological evidence of demyelination. Myelin water fraction increased with remyelination, however MTR remained lower after 28 days. The difference between qT2 and MTR may identify early remyelination.
AB - Identification of remyelination is important in the evaluation of potential treatments of demyelinating diseases such as multiple sclerosis. Local injection of lysolecithin into the brain or spinal cord provides a murine model of demyelination with spontaneous remyelination. The aim of this study was to determine if quantitative, multicomponent T2 (qT2) analysis and magnetization transfer ratio (MTR), both indicative of myelin content, could detect changes in myelination, particularly remyelination, of the cervical spinal cord in mice treated with lysolecithin. We found that the myelin water fraction and geometric mean T2 value of the intra/extracellular water significantly decreased at 14 days then returned to control levels by 28 days after injury, corresponding to clearance of myelin debris and remyelination which was shown by eriochrome cyanine and oil red O staining of histological sections. The MTR was significantly decreased 14 days after lysolecithin injection, and remained low over the time course studied. Evidence of demyelination shown by both qT2 and MTR lagged behind the histological evidence of demyelination. Myelin water fraction increased with remyelination, however MTR remained lower after 28 days. The difference between qT2 and MTR may identify early remyelination.
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U2 - 10.1016/j.neuroimage.2008.12.071
DO - 10.1016/j.neuroimage.2008.12.071
M3 - Article
C2 - 19349232
AN - SCOPUS:62049085008
SN - 1053-8119
VL - 45
SP - 1173
EP - 1182
JO - NeuroImage
JF - NeuroImage
IS - 4
ER -