Multiexcitation fluorescence and reflectance spectroscopy as a real time guide to biopsy in vivo

M. G. Müller, I. Georgakoudi, A. Wax, R. Dasari, C. Jost, M. Wallace, M. S. Feld

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A compact excitation-emission spectrofluorimeter system used to acquire tissue fluorescence and reflectance with fiber optic light delivery and collection is presented. Ten dye cells are pumped by a XeCl excimer laser, creating laser excitation from 308 nm to 505 nm, which is used to excite tissue fluorescence. A xenon-flash lamp is employed to obtain the reflectance spectrum of the corresponding tissue spot. Eleven fluorescence spectra and one white light diffuse reflectance spectrum are collected in vivo in 0.2 seconds and can be potentially analyzed in real time to obtain diagnostic information. The instrument can be used for early cancer detection. The measured fluorescence and reflectance spectra are used to model the intrinsic fluorescence of the tissue, which gives important insight into its biochemical composition (tryptophan, collagen, elastin, NADH, etc.) and metabolic state. Diffuse reflectance provides information about tissue morphology, in terms of the scattering and absorption coefficient. By subtracting the diffusely reflected component from the measured reflectance, light scattering spectroscopic (LSS) information due to single backscattering from epithelial cell nuclei can be obtained. LSS provides information about the size distribution of cell nuclei. The parameters give diagnostic information, which can be used to guide the physician in real time while taking biopsies of invisible tissue abnormalities.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsJ. Conchello, C.J. Cogswell, T. Wilson
Pages114-121
Number of pages8
Volume4261
DOIs
StatePublished - 2001
Externally publishedYes
EventThree-Dimensional and Multidimensional Microscopy: Image Asquisition and Processin VIII - San Jose, CA, United States
Duration: Jan 21 2001Jan 22 2001

Other

OtherThree-Dimensional and Multidimensional Microscopy: Image Asquisition and Processin VIII
CountryUnited States
CitySan Jose, CA
Period1/21/011/22/01

Fingerprint

Biopsy
Fluorescence
Spectroscopy
Tissue
reflectance
fluorescence
spectroscopy
Light scattering
light scattering
elastin
Elastin
xenon lamps
flash lamps
nuclei
Laser excitation
physicians
tryptophan
Xenon
abnormalities
scattering coefficients

Keywords

  • Absorption
  • Excitation emission matrix
  • Fluorescence
  • Intrinsic fluorescence
  • Light scattering spectroscopy
  • Reflectance
  • Scattering
  • Spectroscopy

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Müller, M. G., Georgakoudi, I., Wax, A., Dasari, R., Jost, C., Wallace, M., & Feld, M. S. (2001). Multiexcitation fluorescence and reflectance spectroscopy as a real time guide to biopsy in vivo. In J. Conchello, C. J. Cogswell, & T. Wilson (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4261, pp. 114-121) https://doi.org/10.1117/12.424525

Multiexcitation fluorescence and reflectance spectroscopy as a real time guide to biopsy in vivo. / Müller, M. G.; Georgakoudi, I.; Wax, A.; Dasari, R.; Jost, C.; Wallace, M.; Feld, M. S.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / J. Conchello; C.J. Cogswell; T. Wilson. Vol. 4261 2001. p. 114-121.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Müller, MG, Georgakoudi, I, Wax, A, Dasari, R, Jost, C, Wallace, M & Feld, MS 2001, Multiexcitation fluorescence and reflectance spectroscopy as a real time guide to biopsy in vivo. in J Conchello, CJ Cogswell & T Wilson (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4261, pp. 114-121, Three-Dimensional and Multidimensional Microscopy: Image Asquisition and Processin VIII, San Jose, CA, United States, 1/21/01. https://doi.org/10.1117/12.424525
Müller MG, Georgakoudi I, Wax A, Dasari R, Jost C, Wallace M et al. Multiexcitation fluorescence and reflectance spectroscopy as a real time guide to biopsy in vivo. In Conchello J, Cogswell CJ, Wilson T, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4261. 2001. p. 114-121 https://doi.org/10.1117/12.424525
Müller, M. G. ; Georgakoudi, I. ; Wax, A. ; Dasari, R. ; Jost, C. ; Wallace, M. ; Feld, M. S. / Multiexcitation fluorescence and reflectance spectroscopy as a real time guide to biopsy in vivo. Proceedings of SPIE - The International Society for Optical Engineering. editor / J. Conchello ; C.J. Cogswell ; T. Wilson. Vol. 4261 2001. pp. 114-121
@inproceedings{ecae91729c254023aa799d8112f15a77,
title = "Multiexcitation fluorescence and reflectance spectroscopy as a real time guide to biopsy in vivo",
abstract = "A compact excitation-emission spectrofluorimeter system used to acquire tissue fluorescence and reflectance with fiber optic light delivery and collection is presented. Ten dye cells are pumped by a XeCl excimer laser, creating laser excitation from 308 nm to 505 nm, which is used to excite tissue fluorescence. A xenon-flash lamp is employed to obtain the reflectance spectrum of the corresponding tissue spot. Eleven fluorescence spectra and one white light diffuse reflectance spectrum are collected in vivo in 0.2 seconds and can be potentially analyzed in real time to obtain diagnostic information. The instrument can be used for early cancer detection. The measured fluorescence and reflectance spectra are used to model the intrinsic fluorescence of the tissue, which gives important insight into its biochemical composition (tryptophan, collagen, elastin, NADH, etc.) and metabolic state. Diffuse reflectance provides information about tissue morphology, in terms of the scattering and absorption coefficient. By subtracting the diffusely reflected component from the measured reflectance, light scattering spectroscopic (LSS) information due to single backscattering from epithelial cell nuclei can be obtained. LSS provides information about the size distribution of cell nuclei. The parameters give diagnostic information, which can be used to guide the physician in real time while taking biopsies of invisible tissue abnormalities.",
keywords = "Absorption, Excitation emission matrix, Fluorescence, Intrinsic fluorescence, Light scattering spectroscopy, Reflectance, Scattering, Spectroscopy",
author = "M{\"u}ller, {M. G.} and I. Georgakoudi and A. Wax and R. Dasari and C. Jost and M. Wallace and Feld, {M. S.}",
year = "2001",
doi = "10.1117/12.424525",
language = "English (US)",
volume = "4261",
pages = "114--121",
editor = "J. Conchello and C.J. Cogswell and T. Wilson",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

}

TY - GEN

T1 - Multiexcitation fluorescence and reflectance spectroscopy as a real time guide to biopsy in vivo

AU - Müller, M. G.

AU - Georgakoudi, I.

AU - Wax, A.

AU - Dasari, R.

AU - Jost, C.

AU - Wallace, M.

AU - Feld, M. S.

PY - 2001

Y1 - 2001

N2 - A compact excitation-emission spectrofluorimeter system used to acquire tissue fluorescence and reflectance with fiber optic light delivery and collection is presented. Ten dye cells are pumped by a XeCl excimer laser, creating laser excitation from 308 nm to 505 nm, which is used to excite tissue fluorescence. A xenon-flash lamp is employed to obtain the reflectance spectrum of the corresponding tissue spot. Eleven fluorescence spectra and one white light diffuse reflectance spectrum are collected in vivo in 0.2 seconds and can be potentially analyzed in real time to obtain diagnostic information. The instrument can be used for early cancer detection. The measured fluorescence and reflectance spectra are used to model the intrinsic fluorescence of the tissue, which gives important insight into its biochemical composition (tryptophan, collagen, elastin, NADH, etc.) and metabolic state. Diffuse reflectance provides information about tissue morphology, in terms of the scattering and absorption coefficient. By subtracting the diffusely reflected component from the measured reflectance, light scattering spectroscopic (LSS) information due to single backscattering from epithelial cell nuclei can be obtained. LSS provides information about the size distribution of cell nuclei. The parameters give diagnostic information, which can be used to guide the physician in real time while taking biopsies of invisible tissue abnormalities.

AB - A compact excitation-emission spectrofluorimeter system used to acquire tissue fluorescence and reflectance with fiber optic light delivery and collection is presented. Ten dye cells are pumped by a XeCl excimer laser, creating laser excitation from 308 nm to 505 nm, which is used to excite tissue fluorescence. A xenon-flash lamp is employed to obtain the reflectance spectrum of the corresponding tissue spot. Eleven fluorescence spectra and one white light diffuse reflectance spectrum are collected in vivo in 0.2 seconds and can be potentially analyzed in real time to obtain diagnostic information. The instrument can be used for early cancer detection. The measured fluorescence and reflectance spectra are used to model the intrinsic fluorescence of the tissue, which gives important insight into its biochemical composition (tryptophan, collagen, elastin, NADH, etc.) and metabolic state. Diffuse reflectance provides information about tissue morphology, in terms of the scattering and absorption coefficient. By subtracting the diffusely reflected component from the measured reflectance, light scattering spectroscopic (LSS) information due to single backscattering from epithelial cell nuclei can be obtained. LSS provides information about the size distribution of cell nuclei. The parameters give diagnostic information, which can be used to guide the physician in real time while taking biopsies of invisible tissue abnormalities.

KW - Absorption

KW - Excitation emission matrix

KW - Fluorescence

KW - Intrinsic fluorescence

KW - Light scattering spectroscopy

KW - Reflectance

KW - Scattering

KW - Spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=0034946685&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034946685&partnerID=8YFLogxK

U2 - 10.1117/12.424525

DO - 10.1117/12.424525

M3 - Conference contribution

VL - 4261

SP - 114

EP - 121

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Conchello, J.

A2 - Cogswell, C.J.

A2 - Wilson, T.

ER -