Multicenter performance assessment of Carba NP test

Scott A. Cunningham, Brandi Limbago, Maria Traczewski, Karen Anderson, Meredith Hackel, Janet Hindler, Dan Sahm, Efe Alyanak, Adrian Lawsin, Christopher A. Gulvik, Tom J.B. De Man, Jayawant N. Mandrekar, Audrey N. Schuetz, Stephen Jenkins, Romney Humphries, Elizabeth Palavecino, Shawn Vasoo, Robin Patel

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Eighty Gram-negative bacilli (54 Enterobacteriaceae and 26 nonfermenting Gram-negative bacilli) obtained from multiple institutions in the United States were distributed in a blinded manner to seven testing laboratories to compare their performance of a test for detection of carbapenemase production, the Carba NP test. The Carba NP test was performed by all laboratories, following the Clinical and Laboratory Standards Institute (CLSI) procedure. Site-versus-site comparisons demonstrated a high level of consistency for the Carba NP assay, with just 3/21 site comparisons yielding a difference in sensitivity (P < 0.05). Previously described limitations with blaOXA-48-like carbapenemases and blaOXA carbapenemases associated with Acinetobacter baumannii were noted. Based on these data, we demonstrate that the Carba NP test, when implemented with the standardized CLSI methodology, provides reproducible results across multiple sites for detection of carbapenemases.

Original languageEnglish (US)
Pages (from-to)1954-1960
Number of pages7
JournalJournal of clinical microbiology
Volume55
Issue number6
DOIs
StatePublished - Jun 2017

Keywords

  • Antimicrobial resistance
  • Carbapenemase
  • Gram-negative bacilli
  • Screening

ASJC Scopus subject areas

  • Microbiology (medical)

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    Cunningham, S. A., Limbago, B., Traczewski, M., Anderson, K., Hackel, M., Hindler, J., Sahm, D., Alyanak, E., Lawsin, A., Gulvik, C. A., De Man, T. J. B., Mandrekar, J. N., Schuetz, A. N., Jenkins, S., Humphries, R., Palavecino, E., Vasoo, S., & Patel, R. (2017). Multicenter performance assessment of Carba NP test. Journal of clinical microbiology, 55(6), 1954-1960. https://doi.org/10.1128/JCM.00244-17