TY - JOUR
T1 - Multi-omics reveals microbiome, host gene expression, and immune landscape in gastric carcinogenesis
AU - Park, Chan Hyuk
AU - Hong, Changjin
AU - Lee, A. reum
AU - Sung, Jaeyun
AU - Hwang, Tae Hyun
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2021R1C1C1005728 ) (C.H.P.). The biospecimens and clinical information in the surgery cohort were provided by the Biobank of Keimyung University Dongsan Hospital and Inje University Busan Paik Hospital, who are institutional members of the Korea Biobank Network. Graphical abstract and Figure 1 were created with BioRender.com .
Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2021R1C1C1005728) (C.H.P.). The biospecimens and clinical information in the surgery cohort were provided by the Biobank of Keimyung University Dongsan Hospital and Inje University Busan Paik Hospital, who are institutional members of the Korea Biobank Network. Graphical abstract and Figure 1 were created with BioRender.com. Conceptualization, C.H.P. and T.H.H.; Methodology, C.H.P. C.H. and T.H.H.; Investigation, C.H.P. C.H. and A.L.; Writing ? Original Draft, C.H.P.; Writing ? Review & Editing, C.H.P. C.H. A.L. J.S. and T.H.H.; Funding Acquisition, C.H.P.; Resources, C.H.P. C.H. and T.H.H; Supervision, J.S. and T.H.H. No disclosures were reported.
Publisher Copyright:
© 2022 The Author(s)
PY - 2022/3/18
Y1 - 2022/3/18
N2 - To date, there has been no multi-omic analysis characterizing the intricate relationships between the intragastric microbiome and gastric mucosal gene expression in gastric carcinogenesis. Using multi-omic approaches, we provide a comprehensive view of the connections between the microbiome and host gene expression in distinct stages of gastric carcinogenesis (i.e., healthy, gastritis, cancer). Our integrative analysis uncovers various associations specific to disease states. For example, uniquely in gastritis, Helicobacteraceae is highly correlated with the expression of FAM3D, which has been previously implicated in gastrointestinal inflammation. In addition, in gastric cancer but not in adjacent gastritis, Lachnospiraceae is highly correlated with the expression of UBD, which regulates mitosis and cell cycle time. Furthermore, lower abundances of B cell signatures in gastric cancer compared to gastritis may suggest a previously unidentified immune evasion process in gastric carcinogenesis. Our study provides the most comprehensive description of microbial, host transcriptomic, and immune cell factors of the gastric carcinogenesis pathway.
AB - To date, there has been no multi-omic analysis characterizing the intricate relationships between the intragastric microbiome and gastric mucosal gene expression in gastric carcinogenesis. Using multi-omic approaches, we provide a comprehensive view of the connections between the microbiome and host gene expression in distinct stages of gastric carcinogenesis (i.e., healthy, gastritis, cancer). Our integrative analysis uncovers various associations specific to disease states. For example, uniquely in gastritis, Helicobacteraceae is highly correlated with the expression of FAM3D, which has been previously implicated in gastrointestinal inflammation. In addition, in gastric cancer but not in adjacent gastritis, Lachnospiraceae is highly correlated with the expression of UBD, which regulates mitosis and cell cycle time. Furthermore, lower abundances of B cell signatures in gastric cancer compared to gastritis may suggest a previously unidentified immune evasion process in gastric carcinogenesis. Our study provides the most comprehensive description of microbial, host transcriptomic, and immune cell factors of the gastric carcinogenesis pathway.
KW - Cellular physiology
KW - Microbiome
KW - Omics
UR - http://www.scopus.com/inward/record.url?scp=85125675767&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85125675767&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2022.103956
DO - 10.1016/j.isci.2022.103956
M3 - Article
AN - SCOPUS:85125675767
SN - 2589-0042
VL - 25
JO - iScience
JF - iScience
IS - 3
M1 - 103956
ER -