MUC1 epithelial mucin (CD227) is expressed by activated dendritic cells

M. Wykes, K. P.A. MacDonald, M. Tran, R. J. Quin, P. X. Xing, S. J. Gendler, D. N.J. Hart, Michael A. McGuckin

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

The MUC1 mucin (CD227) is a cell surface mucin originally thought to be restricted to epithelial tissues. We report that CD227 is expressed on human blood dendritic cells (DC) and monocyte-derived DC following in vitro activation. Freshly isolated murine splenic DC had very low levels of CD227; however, all DC expressed CD227 following in vitro culture. In the mouse spleen, CD227 was seen on clusters within the red pulp and surrounding the marginal zone in the white pulp. Additionally, we confirm CD227 expression by activated human T cells and show for the first time that the CD227 cytoplasmic domain is tyrosine-phosphorylated in activated T cells and DC and is associated with other phosphoproteins, indicating a role in signaling. The function of CD227 on DC and T cells requires further elucidation.

Original languageEnglish (US)
Pages (from-to)692-701
Number of pages10
JournalJournal of Leukocyte Biology
Volume72
Issue number4
StatePublished - Oct 1 2002

Keywords

  • Activation
  • Glycoprotein
  • Sialomucin
  • T cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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    Wykes, M., MacDonald, K. P. A., Tran, M., Quin, R. J., Xing, P. X., Gendler, S. J., Hart, D. N. J., & McGuckin, M. A. (2002). MUC1 epithelial mucin (CD227) is expressed by activated dendritic cells. Journal of Leukocyte Biology, 72(4), 692-701.