@article{eaac2999d13549c488e2a45383cf0ca2,
title = "Mps1 kinase-dependent Sgo2 centromere localisation mediates cohesin protection in mouse oocyte meiosis i",
abstract = "A key feature of meiosis is the step-wise removal of cohesin, the protein complex holding sister chromatids together, first from arms in meiosis I and then from the centromere region in meiosis II. Centromeric cohesin is protected by Sgo2 from Separase-mediated cleavage, in order to maintain sister chromatids together until their separation in meiosis II. Failures in step-wise cohesin removal result in aneuploid gametes, preventing the generation of healthy embryos. Here, we report that kinase activities of Bub1 and Mps1 are required for Sgo2 localisation to the centromere region. Mps1 inhibitor-treated oocytes are defective in centromeric cohesin protection, whereas oocytes devoid of Bub1 kinase activity, which cannot phosphorylate H2A at T121, are not perturbed in cohesin protection as long as Mps1 is functional. Mps1 and Bub1 kinase activities localise Sgo2 in meiosis I preferentially to the centromere and pericentromere respectively, indicating that Sgo2 at the centromere is required for protection.",
author = "{El Yakoubi}, Warif and Eulalie Buffin and Damien Cladi{\`e}re and Yulia Gryaznova and In{\'e}s Berenguer and Touati, {Sandra A.} and Roc{\'i}o G{\'o}mez and Suja, {Jos{\'e} A.} and {Van Deursen}, {Jan M.} and Katja Wassmann",
note = "Funding Information: We are grateful to Jos{\'e} Luis Barbero for Sgo2 antibody, Stephen Taylor for Bub1 antibody, Darren J. Baker for help with genotyping, and Hongtao Yu for advice and Mps1 antibody. P.K. Sorger provided the Mps1ΔN strain. We thank E. Nikalayevich, Nora Bouftas, and Antoine Vallot for comments and stimulating discussions, Mathilde R{\'e}cipon, Ioanna Leontiou and Khaled Hached for preliminary experiments, and Vincent Galy for advice on spinning disc confocal microscopy. We furthermore acknowledge help from our colleagues from the animal facility and administration (UMR7622), and Sus-anne Bolte and Jean-Fran{\c c}ois Gilles from the IBPS imaging core facility for help with Confocal microscopy and 3D rendering. W.E.Y. is the recipient of a postdoctoral fellowship by ARC (Association de la Recherche Contre le Cancer), and I.B. of a 5 months visiting Predoctoral Mobility fellowship from the Ministerio de Economia y Competi-tividad, Spain (EEBB-I-14-08883). The lab of K.W. obtained financial support through grants by the Agence Nationale de la Recherche (ANR-12-BSV2-0005-01 and ANR-16-CE92-0007-01 to K.W.), a grant {\textquoteleft}Equipe FRM{\textquoteright} by the Fondation de la Recherche M{\'e}dicale (Equipe DEQ20160334921 to K.W.), the UPMC and CNRS. The group of J.A.S. obtained support through the research project BFU2014-53681-P from the Ministerio de Economia y Competitividad (Spain). Publisher Copyright: {\textcopyright} 2017 The Author(s).",
year = "2017",
month = dec,
day = "1",
doi = "10.1038/s41467-017-00774-3",
language = "English (US)",
volume = "8",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}