Mouse Mucin 1 (MUC1) defined by monoclonal antibodies

Pei Xiang Xing, Catherine Lees, Jodie Lodding, Julie Prenzoska, Georgia Poulos, Mauro Sandrin, Sandra J Gendler, Ian F C Mckenzie

Research output: Contribution to journalArticle

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Abstract

Mucins are highly expressed in many different human cancers and numerous murine monoclonal antibodies (MAbs) to human mucins, particularly Mucin 1 (MUC1), have been produced. However, no such antibodies to murine mucin 1 (muc1) have been described and we now describe 6 different antibodies produced to murine muc1 and to human MUC1 cytoplasmic tail, either by immunising rats, or muc1 o/o mice with synthetic peptides or a fusion protein composed of glutathione-s-transferase (GST) linked to the tandem repeat region of muc1. The antibodies to both the extracellular tandem repeat region and to the cytoplasmic tail were found to react with mucin-containing murine tissues such as breast, stomach, colon, ovary, kidney and pancreas, and the staining patterns were similar to those found in humans. The reagents reacted specifically with muc1 peptides and tissues; however, some cross reactivity with other mucin-derived peptides was noted, particularly those containing the amino acid sequence TSS. Three different epitopes (TSS, TAVLSGTS and LSGTSSP) of the M30, M70 and MFP25 MAbs were detected. Of interest was the finding that some of the antibodies reacted with murine lymphocytes; it was not clear whether these reactions were due to mucin 1 on mouse lymphocytes (MUC1 was considered to be absent from human lymphocyte), or due to cross reaction with a sialic adhesion molecule on lymphocytes. The antibodies should prove valuable reagents when studying differentiation and expression in murine glandular tissues and the ontogeny of mucin-secreting tumours.

Original languageEnglish (US)
Pages (from-to)875-883
Number of pages9
JournalInternational Journal of Cancer
Volume76
Issue number6
DOIs
StatePublished - Jun 10 1998

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Mucin-1
Monoclonal Antibodies
Mucins
Antibodies
Lymphocytes
Tandem Repeat Sequences
Peptides
Tail
Cross Reactions
Glutathione Transferase
Saliva
Epitopes
Pancreas
Amino Acid Sequence
Ovary
Neoplasms
Stomach
Colon
Breast
Staining and Labeling

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Xing, P. X., Lees, C., Lodding, J., Prenzoska, J., Poulos, G., Sandrin, M., ... Mckenzie, I. F. C. (1998). Mouse Mucin 1 (MUC1) defined by monoclonal antibodies. International Journal of Cancer, 76(6), 875-883. https://doi.org/10.1002/(SICI)1097-0215(19980610)76:6<875::AID-IJC18>3.0.CO;2-1

Mouse Mucin 1 (MUC1) defined by monoclonal antibodies. / Xing, Pei Xiang; Lees, Catherine; Lodding, Jodie; Prenzoska, Julie; Poulos, Georgia; Sandrin, Mauro; Gendler, Sandra J; Mckenzie, Ian F C.

In: International Journal of Cancer, Vol. 76, No. 6, 10.06.1998, p. 875-883.

Research output: Contribution to journalArticle

Xing, PX, Lees, C, Lodding, J, Prenzoska, J, Poulos, G, Sandrin, M, Gendler, SJ & Mckenzie, IFC 1998, 'Mouse Mucin 1 (MUC1) defined by monoclonal antibodies', International Journal of Cancer, vol. 76, no. 6, pp. 875-883. https://doi.org/10.1002/(SICI)1097-0215(19980610)76:6<875::AID-IJC18>3.0.CO;2-1
Xing, Pei Xiang ; Lees, Catherine ; Lodding, Jodie ; Prenzoska, Julie ; Poulos, Georgia ; Sandrin, Mauro ; Gendler, Sandra J ; Mckenzie, Ian F C. / Mouse Mucin 1 (MUC1) defined by monoclonal antibodies. In: International Journal of Cancer. 1998 ; Vol. 76, No. 6. pp. 875-883.
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