Mouse monoclonal antibodies generated from full length human cereblon: Detection of cereblon protein in patients with multiple myeloma

Xiubao Chang, Qinqin Xu, Yuexian Hou, Cynthia Li, Ye Xu, A. Keith Stewart

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Immunomodulatory drugs (IMiDs) are profoundly active compounds in the treatment of patients with multiple myeloma (MM). However, despite the fact that treatment with IMiDs has dramatically improved survival for patients with MM, the majority of MM patients develop IMiDs resistance over time. We have found that expression of functional cereblon is required for IMiDs’ action. In addition, it has been reported that cells expressing high levels of cereblon are resistant to proteasome inhibitor, implying that patients with high levels of cereblon should be resistant to proteasome inhibitor. If the above conclusions are correct, cereblon could be considered as a biomarker to determine which standard regimens should be used to treat patients with MM. Unfortunately, the conclusions mentioned above have not been clinically confirmed. In order to confirm these conclusions, we have generated three highly specific mouse monoclonal antibodies (mAbs) against full-length human cereblon. These mAbs can be used to do western blot, immunoprecipitation and immunohistochemistry staining. In addition, their epitopes have been precisely determined and the peptides covering their epitopes completely blocked the antibody binding to cereblon in western blot analysis or in immunohistochemistry staining of MM patients’ specimens.

Original languageEnglish (US)
Article number1999
JournalInternational journal of molecular sciences
Volume18
Issue number9
DOIs
StatePublished - Sep 17 2017

Keywords

  • Cereblon (CRBN)
  • Epitope
  • Immunohistochemistry (IHC) staining
  • Immunomodulatory drugs (IMiDs)
  • Immunoprecipitation
  • Monoclonal antibody (mAb)
  • Multiple myeloma (MM)

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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