Mouse eosinophils possess potent antibacterial properties in vivo

Stefanie N. Linch, Ann M. Kelly, Erin T. Danielson, Ralph Pero, James J. Lee, Jeffrey A. Gold

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Eosinophils are best known as the predominant cellular infiltrate associated with asthma and parasitic infections. Recently, numerous studies have documented the presence of Toll-like receptors (TLRs) on the surfaces of eosinophils, suggesting that these leukocytes may participate in the recognition and killing of viruses and bacteria. However, the significance of this role in the innate immune response to bacterial infection is largely unknown. Here we report a novel role for eosinophils as antibacterial defenders in the host response. Isolated mouse eosinophils possessed antipseudomonal properties in vitro. In vivo, interleukin-5 transgenic mice, which have profound eosinophilia, demonstrated improved clearance of Pseudomonas aeruginosa introduced into the peritoneal cavity. The findings of improved bacterial clearance following adoptive transfer of eosinophils, and impaired bacterial clearance in mice with a congenital eosinophil deficiency, established that this effect was eosinophil specific. The data presented also demonstrate that eosinophils mediate this antibacterial effect in part through the release of cationic secondary granule proteins. Specifically, isolated eosinophil granules had antibacterial properties in vitro, and administration of eosinophil granule extracts significantly improved bacterial clearance in vivo. These data suggest a potent yet underappreciated antibacterial role for eosinophils in vivo, specifically for eosinophil granules. Moreover, the data suggest that the administration of eosinophil-derived products may represent a viable adjuvant therapy for septic or bacteremic patients in the intensive care unit.

Original languageEnglish (US)
Pages (from-to)4976-4982
Number of pages7
JournalInfection and Immunity
Volume77
Issue number11
DOIs
StatePublished - Nov 2009

Fingerprint

Eosinophils
Parasitic Diseases
Adoptive Transfer
Toll-Like Receptors
Interleukin-5
Peritoneal Cavity
Eosinophilia
Bacterial Infections
Innate Immunity
Pseudomonas aeruginosa
Transgenic Mice
Intensive Care Units
Leukocytes
Asthma
Viruses
Bacteria

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Linch, S. N., Kelly, A. M., Danielson, E. T., Pero, R., Lee, J. J., & Gold, J. A. (2009). Mouse eosinophils possess potent antibacterial properties in vivo. Infection and Immunity, 77(11), 4976-4982. https://doi.org/10.1128/IAI.00306-09

Mouse eosinophils possess potent antibacterial properties in vivo. / Linch, Stefanie N.; Kelly, Ann M.; Danielson, Erin T.; Pero, Ralph; Lee, James J.; Gold, Jeffrey A.

In: Infection and Immunity, Vol. 77, No. 11, 11.2009, p. 4976-4982.

Research output: Contribution to journalArticle

Linch, SN, Kelly, AM, Danielson, ET, Pero, R, Lee, JJ & Gold, JA 2009, 'Mouse eosinophils possess potent antibacterial properties in vivo', Infection and Immunity, vol. 77, no. 11, pp. 4976-4982. https://doi.org/10.1128/IAI.00306-09
Linch SN, Kelly AM, Danielson ET, Pero R, Lee JJ, Gold JA. Mouse eosinophils possess potent antibacterial properties in vivo. Infection and Immunity. 2009 Nov;77(11):4976-4982. https://doi.org/10.1128/IAI.00306-09
Linch, Stefanie N. ; Kelly, Ann M. ; Danielson, Erin T. ; Pero, Ralph ; Lee, James J. ; Gold, Jeffrey A. / Mouse eosinophils possess potent antibacterial properties in vivo. In: Infection and Immunity. 2009 ; Vol. 77, No. 11. pp. 4976-4982.
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