Morphometry of dorsal root ganglion in chronic experimental diabetic neuropathy

Motoko Kishi, James Tanabe, James D. Schmelzer, Phillip A. Low

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

Chronic hyperglycemia results in a predominantly sensory neuropathy. Recent studies suggest that dorsal root ganglion (DRG) neurons comprise a specific target and may be responsible for the important complication of diabetic sensory neuropathy, since hyperglycemia for longer than 6 months results in a vacuolar ganglionopathy with associated radiculopathy and distal sensory neuropathy. We undertook morphometric analysis of L5 DRG neurons in seven diabetic rats and six age- and sex-matched littermates. Nerve conduction studies were also performed, and neuropathy was confirmed. Diabetes was induced with streptozotocin; duration of diabetes was 12 months. The DRG count for control rats was 15,304 ± 991 neurons. Two of seven diabetic DRG counts were reduced, but the group mean count at 14,847 ± 1,524 was not significantly reduced. The number of small neurons (type B) considerably exceeded that of large neurons (type A), at a ratio of 71:29. The percentage of large cells was significantly reduced in diabetic compared with control rats (P = 0.01). The large-diameter population can be subdivided into two groups; with this subdivision, the number of neurons <50 μm was not reduced in samples from diabetic rats, but the neurons of largest size (≥50 μm) were significantly reduced (by 41%).

Original languageEnglish (US)
Pages (from-to)819-824
Number of pages6
JournalDiabetes
Volume51
Issue number3
DOIs
StatePublished - Jan 1 2002

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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