Morphogenesis of the retinal pigment epithelium

Toward understanding retinal degenerative diseases

Alan D Marmorstein, Silvia C. Finnemann, Vera L. Bonilha, Enrique Rodriguez-Boulan

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The phenotype of an epithelial cell is defined by a unique combination of morphology, gene and protein expression, and protein localization. Results indicate that the terminal differentiation of the RPE cell can be described in part by changes in the polarity of its surface proteins αvβ5 integrin, Na,K-ATPase, N-CAM, and EMMPRIN. Changes in protein/gene expression and protein localization in late stages of RPE development indentify αvβ5 integrin as a key player in RPE phagocytosis, and N-CAM and EMMPRIN as potentially important molecules in other RPE functions necessary for photoreceptor survival. By studying the trafficking of the later two proteins it is shown that entry into an apical or basolateral pathway in RPE cells cannot be predicted by the distribution of a given protein in other epithelial cells, and that this distribution may change through the course of RPE development. The mechanisms used by RPE and other epithelia to establish and maintain their specific polarity properties are fundamental to the formation and maintenance of their specific epithelial phenotype. The ability to therapeutically direct molecules incorporated into RPE by gene therapy into apical or basal surfaces requires an understanding of protein localization and expression. Furthermore, evidence is provided that assays capitalizing on changes in gene/protein expression and protein localization during the late stages of RPE development can prove a productive way of identifying proteins used by RPE for photoreceptor support. This approach can continue to be exploited to identify other proteins essential for the mission of the RPE cell, that may thus be likely candidates for participation in retinal degenerative disease.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalAnnals of the New York Academy of Sciences
Volume857
StatePublished - 1998
Externally publishedYes

Fingerprint

Retinal Diseases
Retinal Pigments
Retinal Pigment Epithelium
Morphogenesis
Proteins
Computer aided manufacturing
Integrins
Gene Expression
Protein
Pigments
Epithelial Cells
Gene therapy
Molecules
Phenotype
Gene expression
Phagocytosis
Adenosine Triphosphatases
Genetic Therapy
Assays
Membrane Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Morphogenesis of the retinal pigment epithelium : Toward understanding retinal degenerative diseases. / Marmorstein, Alan D; Finnemann, Silvia C.; Bonilha, Vera L.; Rodriguez-Boulan, Enrique.

In: Annals of the New York Academy of Sciences, Vol. 857, 1998, p. 1-12.

Research output: Contribution to journalArticle

Marmorstein, Alan D ; Finnemann, Silvia C. ; Bonilha, Vera L. ; Rodriguez-Boulan, Enrique. / Morphogenesis of the retinal pigment epithelium : Toward understanding retinal degenerative diseases. In: Annals of the New York Academy of Sciences. 1998 ; Vol. 857. pp. 1-12.
@article{893ab1b62f5d4715abb70e0e8d3ccae0,
title = "Morphogenesis of the retinal pigment epithelium: Toward understanding retinal degenerative diseases",
abstract = "The phenotype of an epithelial cell is defined by a unique combination of morphology, gene and protein expression, and protein localization. Results indicate that the terminal differentiation of the RPE cell can be described in part by changes in the polarity of its surface proteins αvβ5 integrin, Na,K-ATPase, N-CAM, and EMMPRIN. Changes in protein/gene expression and protein localization in late stages of RPE development indentify αvβ5 integrin as a key player in RPE phagocytosis, and N-CAM and EMMPRIN as potentially important molecules in other RPE functions necessary for photoreceptor survival. By studying the trafficking of the later two proteins it is shown that entry into an apical or basolateral pathway in RPE cells cannot be predicted by the distribution of a given protein in other epithelial cells, and that this distribution may change through the course of RPE development. The mechanisms used by RPE and other epithelia to establish and maintain their specific polarity properties are fundamental to the formation and maintenance of their specific epithelial phenotype. The ability to therapeutically direct molecules incorporated into RPE by gene therapy into apical or basal surfaces requires an understanding of protein localization and expression. Furthermore, evidence is provided that assays capitalizing on changes in gene/protein expression and protein localization during the late stages of RPE development can prove a productive way of identifying proteins used by RPE for photoreceptor support. This approach can continue to be exploited to identify other proteins essential for the mission of the RPE cell, that may thus be likely candidates for participation in retinal degenerative disease.",
author = "Marmorstein, {Alan D} and Finnemann, {Silvia C.} and Bonilha, {Vera L.} and Enrique Rodriguez-Boulan",
year = "1998",
language = "English (US)",
volume = "857",
pages = "1--12",
journal = "Annals of the New York Academy of Sciences",
issn = "0077-8923",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Morphogenesis of the retinal pigment epithelium

T2 - Toward understanding retinal degenerative diseases

AU - Marmorstein, Alan D

AU - Finnemann, Silvia C.

AU - Bonilha, Vera L.

AU - Rodriguez-Boulan, Enrique

PY - 1998

Y1 - 1998

N2 - The phenotype of an epithelial cell is defined by a unique combination of morphology, gene and protein expression, and protein localization. Results indicate that the terminal differentiation of the RPE cell can be described in part by changes in the polarity of its surface proteins αvβ5 integrin, Na,K-ATPase, N-CAM, and EMMPRIN. Changes in protein/gene expression and protein localization in late stages of RPE development indentify αvβ5 integrin as a key player in RPE phagocytosis, and N-CAM and EMMPRIN as potentially important molecules in other RPE functions necessary for photoreceptor survival. By studying the trafficking of the later two proteins it is shown that entry into an apical or basolateral pathway in RPE cells cannot be predicted by the distribution of a given protein in other epithelial cells, and that this distribution may change through the course of RPE development. The mechanisms used by RPE and other epithelia to establish and maintain their specific polarity properties are fundamental to the formation and maintenance of their specific epithelial phenotype. The ability to therapeutically direct molecules incorporated into RPE by gene therapy into apical or basal surfaces requires an understanding of protein localization and expression. Furthermore, evidence is provided that assays capitalizing on changes in gene/protein expression and protein localization during the late stages of RPE development can prove a productive way of identifying proteins used by RPE for photoreceptor support. This approach can continue to be exploited to identify other proteins essential for the mission of the RPE cell, that may thus be likely candidates for participation in retinal degenerative disease.

AB - The phenotype of an epithelial cell is defined by a unique combination of morphology, gene and protein expression, and protein localization. Results indicate that the terminal differentiation of the RPE cell can be described in part by changes in the polarity of its surface proteins αvβ5 integrin, Na,K-ATPase, N-CAM, and EMMPRIN. Changes in protein/gene expression and protein localization in late stages of RPE development indentify αvβ5 integrin as a key player in RPE phagocytosis, and N-CAM and EMMPRIN as potentially important molecules in other RPE functions necessary for photoreceptor survival. By studying the trafficking of the later two proteins it is shown that entry into an apical or basolateral pathway in RPE cells cannot be predicted by the distribution of a given protein in other epithelial cells, and that this distribution may change through the course of RPE development. The mechanisms used by RPE and other epithelia to establish and maintain their specific polarity properties are fundamental to the formation and maintenance of their specific epithelial phenotype. The ability to therapeutically direct molecules incorporated into RPE by gene therapy into apical or basal surfaces requires an understanding of protein localization and expression. Furthermore, evidence is provided that assays capitalizing on changes in gene/protein expression and protein localization during the late stages of RPE development can prove a productive way of identifying proteins used by RPE for photoreceptor support. This approach can continue to be exploited to identify other proteins essential for the mission of the RPE cell, that may thus be likely candidates for participation in retinal degenerative disease.

UR - http://www.scopus.com/inward/record.url?scp=0031761418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031761418&partnerID=8YFLogxK

M3 - Article

VL - 857

SP - 1

EP - 12

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

SN - 0077-8923

ER -