Morphants

A new systematic vertebrate functional genomics approach

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

The vertebrate genome contains a predicted 50000-100000 genes, many of unknown function. The recent development of morpholino-based gene knock-down technology in zebraflsh has opened the door to the genome-wide assignment of function based on sequence in a model vertebrate. This review describes technical aspects of morpholino use for functional genomics applications, including the potential for multigene targeting and known methodological limitations. The result of successful gene inactivation by this agent is proposed to yield embryos with a 'morphant' phenotypic designation. The establishment of a morphant database opens the door to true functional genomics using the vertebrate, Danio redo.

Original languageEnglish (US)
Pages (from-to)302-306
Number of pages5
JournalYeast
Volume17
Issue number4
StatePublished - 2000
Externally publishedYes

Fingerprint

Genomics
Vertebrates
Morpholinos
Genes
vertebrates
genomics
Danio
Genome
Gene Knockdown Techniques
genome
genes
Gene Silencing
inactivation
embryo (animal)
Embryonic Structures
Databases
Technology

Keywords

  • Functional genomics
  • Morphant
  • Morpholino
  • Vertebrate
  • Zebrafish

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Morphants : A new systematic vertebrate functional genomics approach. / Ekker, Stephen C.

In: Yeast, Vol. 17, No. 4, 2000, p. 302-306.

Research output: Contribution to journalArticle

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