Morphants: A new systematic vertebrate functional genomics approach

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132 Scopus citations

Abstract

The vertebrate genome contains a predicted 50000-100000 genes, many of unknown function. The recent development of morpholino-based gene knock-down technology in zebraflsh has opened the door to the genome-wide assignment of function based on sequence in a model vertebrate. This review describes technical aspects of morpholino use for functional genomics applications, including the potential for multigene targeting and known methodological limitations. The result of successful gene inactivation by this agent is proposed to yield embryos with a 'morphant' phenotypic designation. The establishment of a morphant database opens the door to true functional genomics using the vertebrate, Danio redo.

Original languageEnglish (US)
Pages (from-to)302-306
Number of pages5
JournalYeast
Volume17
Issue number4
DOIs
StatePublished - 2000

Keywords

  • Functional genomics
  • Morphant
  • Morpholino
  • Vertebrate
  • Zebrafish

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

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