TY - JOUR
T1 - More insights into a human adipose tissue GPAT activity assay
AU - Morgan-Bathke, Maria
AU - Chen, Liang
AU - Oberschneider, Elisabeth
AU - Harteneck, Debra
AU - Jensen, Michael D.
N1 - Publisher Copyright:
© 2016 Taylor & Francis Group, LLC.
PY - 2016/2/3
Y1 - 2016/2/3
N2 - ABSTRACT: Adipose tissue fatty acid storage varies according to sex, adipose tissue depot and degree of fat gain. However, the mechanism(s) for these variations is not completely understood. We recently published findings based on the glycerol 3-phosphate acyltransferase (GPAT) enzyme activity assay we optimized for use with human adipose tissue. These findings include a decrease in total GPAT and GPAT1 as a function of adipocyte size in both omental and subcutaneous adipose tissue and a strong, positive correlations between ACS, GPAT, and DGAT activities for both sexes and depots and between these storage factors and palmitate storage rates into TAG. The aim of this commentary is to expand upon the data from our recent publication. We describe here additional details on the optimization of the GPAT enzyme activity assay, a correlation between DGAT and percentage palmitate in the diacylglycerol fraction, and sex differences in fatty acid storage factors and storage rates into TAG at high palmitate concentrations.
AB - ABSTRACT: Adipose tissue fatty acid storage varies according to sex, adipose tissue depot and degree of fat gain. However, the mechanism(s) for these variations is not completely understood. We recently published findings based on the glycerol 3-phosphate acyltransferase (GPAT) enzyme activity assay we optimized for use with human adipose tissue. These findings include a decrease in total GPAT and GPAT1 as a function of adipocyte size in both omental and subcutaneous adipose tissue and a strong, positive correlations between ACS, GPAT, and DGAT activities for both sexes and depots and between these storage factors and palmitate storage rates into TAG. The aim of this commentary is to expand upon the data from our recent publication. We describe here additional details on the optimization of the GPAT enzyme activity assay, a correlation between DGAT and percentage palmitate in the diacylglycerol fraction, and sex differences in fatty acid storage factors and storage rates into TAG at high palmitate concentrations.
KW - DGAT
KW - GPAT
KW - fat distribution
KW - fatty acid storage
KW - omental adipose tissue
KW - subcutaneous adipose tissue
KW - triglyceride synthesis
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U2 - 10.1080/21623945.2015.1068977
DO - 10.1080/21623945.2015.1068977
M3 - Comment/debate
AN - SCOPUS:85058337512
SN - 2162-3945
VL - 5
SP - 93
EP - 96
JO - Adipocyte
JF - Adipocyte
IS - 1
ER -