More insights into a human adipose tissue GPAT activity assay

Maria Morgan-Bathke, Liang Chen, Elisabeth Oberschneider, Debra Harteneck, Michael Dennis Jensen

Research output: Contribution to journalComment/debate

3 Scopus citations

Abstract

ABSTRACT: Adipose tissue fatty acid storage varies according to sex, adipose tissue depot and degree of fat gain. However, the mechanism(s) for these variations is not completely understood. We recently published findings based on the glycerol 3-phosphate acyltransferase (GPAT) enzyme activity assay we optimized for use with human adipose tissue. These findings include a decrease in total GPAT and GPAT1 as a function of adipocyte size in both omental and subcutaneous adipose tissue and a strong, positive correlations between ACS, GPAT, and DGAT activities for both sexes and depots and between these storage factors and palmitate storage rates into TAG. The aim of this commentary is to expand upon the data from our recent publication. We describe here additional details on the optimization of the GPAT enzyme activity assay, a correlation between DGAT and percentage palmitate in the diacylglycerol fraction, and sex differences in fatty acid storage factors and storage rates into TAG at high palmitate concentrations.

Original languageEnglish (US)
Pages (from-to)93-96
Number of pages4
JournalAdipocyte
Volume5
Issue number1
DOIs
StatePublished - Feb 3 2016

Keywords

  • DGAT
  • fat distribution
  • fatty acid storage
  • GPAT
  • omental adipose tissue
  • subcutaneous adipose tissue
  • triglyceride synthesis

ASJC Scopus subject areas

  • Medicine(all)

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