Abstract
The disaccharides trehalose and sucrose, and to a lesser extent the monosaccharides glucose and fructose, decrease the apparent Kmof the Ca2+,Mg2+-ATPase of sarcoplasmic reticulum for Pi. This effect is more pronounced at pH 7.4 than at pH 6.2. The enzyme is not phosphorylated by Pi when the temperature of the medium is decreased to 0 °C, but when 1.5 m trehaloseor sucrose is present phosphoenzyme formation increases to 0.5 μmol E-P/g protein.
Original language | English (US) |
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Pages (from-to) | 644-646 |
Number of pages | 3 |
Journal | Zeitschrift fur Naturforschung - Section C Journal of Biosciences |
Volume | 46 |
Issue number | 7-8 |
DOIs | |
State | Published - Aug 1991 |
Keywords
- Enzyme (Ca2+
- Mg2+) ATPase (EC 3.6.1.38)
- Phosphorylation
- Saccharides
- Sarcoplasmic Reticulum
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology