Monosaccharides and Disaccharides Decrease the Kmfor Phosphorylation of a Membrane-Bound Enzyme ATPase

Eduardo N. Chini, Jose Roberto Meyer-Fernandes, Mauro Sola-Penna

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The disaccharides trehalose and sucrose, and to a lesser extent the monosaccharides glucose and fructose, decrease the apparent Kmof the Ca2+,Mg2+-ATPase of sarcoplasmic reticulum for Pi. This effect is more pronounced at pH 7.4 than at pH 6.2. The enzyme is not phosphorylated by Pi when the temperature of the medium is decreased to 0 °C, but when 1.5 m trehaloseor sucrose is present phosphoenzyme formation increases to 0.5 μmol E-P/g protein.

Original languageEnglish (US)
Pages (from-to)644-646
Number of pages3
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume46
Issue number7-8
DOIs
StatePublished - Aug 1991

Keywords

  • Enzyme (Ca2+
  • Mg2+) ATPase (EC 3.6.1.38)
  • Phosphorylation
  • Saccharides
  • Sarcoplasmic Reticulum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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