Monosaccharides and Disaccharides Decrease the Kmfor Phosphorylation of a Membrane-Bound Enzyme ATPase

Eduardo N. Chini; Jose Roberto Meyer-Fernandes; Mauro Sola-Penna

doi:10.1515/znc-1991-7-822

Monosaccharides and Disaccharides Decrease the K_mfor Phosphorylation of a Membrane-Bound Enzyme ATPase

Eduardo N. Chini, Jose Roberto Meyer-Fernandes, Mauro Sola-Penna

Anesthesiology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The disaccharides trehalose and sucrose, and to a lesser extent the monosaccharides glucose and fructose, decrease the apparent K_mof the Ca²⁺,Mg²⁺-ATPase of sarcoplasmic reticulum for Pi. This effect is more pronounced at pH 7.4 than at pH 6.2. The enzyme is not phosphorylated by Pi when the temperature of the medium is decreased to 0 °C, but when 1.5 _m _trehaloseor sucrose is present phosphoenzyme formation increases to 0.5 μmol E-P/g protein.

Original language	English (US)
Pages (from-to)	644-646
Number of pages	3
Journal	Zeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume	46
Issue number	7-8
DOIs	https://doi.org/10.1515/znc-1991-7-822
State	Published - Aug 1991

Keywords

Enzyme (Ca2+
Mg2+) ATPase (EC 3.6.1.38)
Phosphorylation
Saccharides
Sarcoplasmic Reticulum

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1515/znc-1991-7-822

Cite this

@article{24a79c05afec49ed99453af69d00bcdc,

title = "Monosaccharides and Disaccharides Decrease the Kmfor Phosphorylation of a Membrane-Bound Enzyme ATPase",

abstract = "The disaccharides trehalose and sucrose, and to a lesser extent the monosaccharides glucose and fructose, decrease the apparent Kmof the Ca2+,Mg2+-ATPase of sarcoplasmic reticulum for Pi. This effect is more pronounced at pH 7.4 than at pH 6.2. The enzyme is not phosphorylated by Pi when the temperature of the medium is decreased to 0 °C, but when 1.5 m trehaloseor sucrose is present phosphoenzyme formation increases to 0.5 μmol E-P/g protein.",

keywords = "Enzyme (Ca2+, Mg2+) ATPase (EC 3.6.1.38), Phosphorylation, Saccharides, Sarcoplasmic Reticulum",

author = "Chini, {Eduardo N.} and Meyer-Fernandes, {Jose Roberto} and Mauro Sola-Penna",

note = "Funding Information: E. N. Chini and M. Sola-Penna are recipients of a fellowship from Conselho Nacional de Desen-volvimento Cientifico e Tecnologico (CNPq). We thank Frederico G. Steidel de Toledo, Dr. Leopol-do de Meis and Martha Sorenson for helpful discussions of the manuscript.",

year = "1991",

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doi = "10.1515/znc-1991-7-822",

language = "English (US)",

volume = "46",

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T1 - Monosaccharides and Disaccharides Decrease the Kmfor Phosphorylation of a Membrane-Bound Enzyme ATPase

AU - Chini, Eduardo N.

AU - Meyer-Fernandes, Jose Roberto

AU - Sola-Penna, Mauro

N1 - Funding Information: E. N. Chini and M. Sola-Penna are recipients of a fellowship from Conselho Nacional de Desen-volvimento Cientifico e Tecnologico (CNPq). We thank Frederico G. Steidel de Toledo, Dr. Leopol-do de Meis and Martha Sorenson for helpful discussions of the manuscript.

PY - 1991/8

Y1 - 1991/8

N2 - The disaccharides trehalose and sucrose, and to a lesser extent the monosaccharides glucose and fructose, decrease the apparent Kmof the Ca2+,Mg2+-ATPase of sarcoplasmic reticulum for Pi. This effect is more pronounced at pH 7.4 than at pH 6.2. The enzyme is not phosphorylated by Pi when the temperature of the medium is decreased to 0 °C, but when 1.5 m trehaloseor sucrose is present phosphoenzyme formation increases to 0.5 μmol E-P/g protein.

AB - The disaccharides trehalose and sucrose, and to a lesser extent the monosaccharides glucose and fructose, decrease the apparent Kmof the Ca2+,Mg2+-ATPase of sarcoplasmic reticulum for Pi. This effect is more pronounced at pH 7.4 than at pH 6.2. The enzyme is not phosphorylated by Pi when the temperature of the medium is decreased to 0 °C, but when 1.5 m trehaloseor sucrose is present phosphoenzyme formation increases to 0.5 μmol E-P/g protein.

KW - Enzyme (Ca2+

KW - Mg2+) ATPase (EC 3.6.1.38)

KW - Phosphorylation

KW - Saccharides

KW - Sarcoplasmic Reticulum

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