Monosaccharides and disaccharides decrease the Km for phosphorylation of a membrane-bound enzyme ATPase.

Eduardo Nunes Chini, J. R. Meyer-Fernandes, M. Sola-Penna

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The disaccharides trehalose and sucrose, and to a lesser extent the monosaccharides glucose and fructose, decrease the apparent Km of the Ca2+, Mg(2+)-ATPase of sarcoplasmic reticulum for Pi. This effect is more pronounced at pH 7.4 than at pH 6.2. The enzyme is not phosphorylated by Pi when the temperature of the medium is decreased to 0 degree C, but when 1.5 M trehalose or sucrose is present phosphoenzyme formation increases to 0.5 mumol E-P/g protein.

Original languageEnglish (US)
Pages (from-to)644-646
Number of pages3
JournalZeitschrift fur Naturforschung. C, Journal of biosciences
Volume46
Issue number7-8
StatePublished - Jul 1991
Externally publishedYes

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Trehalose
Phosphorylation
Monosaccharides
Disaccharides
Adenosine Triphosphatases
Sucrose
Membranes
Sarcoplasmic Reticulum
Enzymes
Fructose
Glucose
Temperature
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Monosaccharides and disaccharides decrease the Km for phosphorylation of a membrane-bound enzyme ATPase. / Chini, Eduardo Nunes; Meyer-Fernandes, J. R.; Sola-Penna, M.

In: Zeitschrift fur Naturforschung. C, Journal of biosciences, Vol. 46, No. 7-8, 07.1991, p. 644-646.

Research output: Contribution to journalArticle

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