Monomeric class I molecules mediate TCR/CD3ε/CD8 interaction on the surface of T cells

M. S. Block, A. J. Johnson, Y. Mendez-Fernandez, L. R. Pease

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Both CD8 and the TCR bind to MHC class I molecules during physiologic T cell activation. It has been shown that for optimal T cell activation to occur, CD8 must be able to bind the same class I molecule that is bound by the TCR. However, no direct evidence for the class I-dependent association of CD8 and the TCR has been demonstrated. Using fluorescence resonance energy transfer, we show directly that a single class I molecule causes TCR/CD8 interaction by serving as a docking molecule for both CD8 and the TCR. Furthermore, we show that CD3ε is brought into close proximity with CD8 upon TCR/CD8 association. These interactions are not dependent on the phosphorylation events characteristic of T cell activation. Thus, MHC class I molecules, by binding to both CD8 and the TCR, mediate the reorganization of T cell membrane components to promote cellular activation.

Original languageEnglish (US)
Pages (from-to)821-826
Number of pages6
JournalJournal of Immunology
Volume167
Issue number2
StatePublished - Jul 15 2001

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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