TY - JOUR
T1 - Monomeric class I molecules mediate TCR/CD3ε/CD8 interaction on the surface of T cells
AU - Block, M. S.
AU - Johnson, A. J.
AU - Mendez-Fernandez, Y.
AU - Pease, L. R.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2001/7/15
Y1 - 2001/7/15
N2 - Both CD8 and the TCR bind to MHC class I molecules during physiologic T cell activation. It has been shown that for optimal T cell activation to occur, CD8 must be able to bind the same class I molecule that is bound by the TCR. However, no direct evidence for the class I-dependent association of CD8 and the TCR has been demonstrated. Using fluorescence resonance energy transfer, we show directly that a single class I molecule causes TCR/CD8 interaction by serving as a docking molecule for both CD8 and the TCR. Furthermore, we show that CD3ε is brought into close proximity with CD8 upon TCR/CD8 association. These interactions are not dependent on the phosphorylation events characteristic of T cell activation. Thus, MHC class I molecules, by binding to both CD8 and the TCR, mediate the reorganization of T cell membrane components to promote cellular activation.
AB - Both CD8 and the TCR bind to MHC class I molecules during physiologic T cell activation. It has been shown that for optimal T cell activation to occur, CD8 must be able to bind the same class I molecule that is bound by the TCR. However, no direct evidence for the class I-dependent association of CD8 and the TCR has been demonstrated. Using fluorescence resonance energy transfer, we show directly that a single class I molecule causes TCR/CD8 interaction by serving as a docking molecule for both CD8 and the TCR. Furthermore, we show that CD3ε is brought into close proximity with CD8 upon TCR/CD8 association. These interactions are not dependent on the phosphorylation events characteristic of T cell activation. Thus, MHC class I molecules, by binding to both CD8 and the TCR, mediate the reorganization of T cell membrane components to promote cellular activation.
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U2 - 10.4049/jimmunol.167.2.821
DO - 10.4049/jimmunol.167.2.821
M3 - Article
C2 - 11441088
AN - SCOPUS:0035879126
SN - 0022-1767
VL - 167
SP - 821
EP - 826
JO - Journal of Immunology
JF - Journal of Immunology
IS - 2
ER -