Monocyte HLA-DR expression and neutrophil CD64 expression as biomarkers of infection in critically ill neonates and infants

Justin E. Juskewitch, Roshini S. Abraham, Stacy C. League, Sarah M. Jenkins, Carin Y. Smith, Felicity T. Enders, Stefan K. Grebe, William A. Carey, W. Charles Huskins

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Reduced monocyte HLA-DR expression and increased neutrophil CD64 expression have been proposed as biomarkers of infection.Methods:From 2009-2011, blood samples from neonatal intensive care unit (NICU) and pediatric intensive care unit (ICU) patients <1 y of age were collected at enrollment and during subsequent evaluation for suspected infection, if it occurred. Samples were analyzed for monocyte HLA-DR and neutrophil CD64 expression levels by flow cytometry.Results:Forty-seven infants had study samples collected at enrollment; 26 infants had study samples collected at the time of a suspected infection. At enrollment, there was an inverse relationship between neutrophil CD64 expression and age (P ≤ 0.047). At the time of suspected infection, infants with an infection demonstrated a lower percentage of HLA-DR+ monocytes (P = 0.02, area under the curve (AUC) 0.78), higher percentage of CD64+ neutrophils (P = 0.009, AUC 0.81), and higher neutrophil CD64 expression levels (P = 0.04, AUC 0.75).Conclusion:Monocyte HLA-DR and neutrophil CD64 expression in critically ill infants are related to age and infection.

Original languageEnglish (US)
Pages (from-to)683-690
Number of pages8
JournalPediatric Research
Volume78
Issue number6
DOIs
StatePublished - Dec 1 2015

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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