Monoclonal CLL B-cells may be induced to grow in an in vitro B-cell colony assay system

R. T. Perri, Neil Elliot Kay

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A simple reproducible in vitro B-cell colony assay system was used to evaluate B-cell growth in controls and patients with chronic lymphocytic leukemia (CLL). All six CLL patients studied formed B-cell colonies. The number of colonies was significantly less in patients than controls (66 ± 18 versus 127 ± 8). CLL colonies were shown to be monoclonal and appeared to reflect the circulating malignant B-cell clone in our patient group, while the six controls studied formed polyclonal B-cell colonies. Wright-Giemsa staining showed typical plasma cells to have developed in the controls but not in the patients. Cells from CLL patients retained a more lymphoid appearance. It is believed that investigations with this B-cell assay will provide the means for further in vitro evaluation of malignant B-cell proliferation in other lymphoproliferative disorders.

Original languageEnglish (US)
Pages (from-to)247-249
Number of pages3
JournalBlood
Volume59
Issue number2
StatePublished - 1982
Externally publishedYes

Fingerprint

B-Cell Chronic Lymphocytic Leukemia
Assays
B-Lymphocytes
Cells
Clone cells
Cell proliferation
Cell growth
Lymphoproliferative Disorders
Plasmas
Plasma Cells
In Vitro Techniques
Clone Cells
Cell Proliferation
Staining and Labeling
Growth

ASJC Scopus subject areas

  • Hematology

Cite this

Monoclonal CLL B-cells may be induced to grow in an in vitro B-cell colony assay system. / Perri, R. T.; Kay, Neil Elliot.

In: Blood, Vol. 59, No. 2, 1982, p. 247-249.

Research output: Contribution to journalArticle

@article{c2f9bd4e0f9c4019bade4cce95141fca,
title = "Monoclonal CLL B-cells may be induced to grow in an in vitro B-cell colony assay system",
abstract = "A simple reproducible in vitro B-cell colony assay system was used to evaluate B-cell growth in controls and patients with chronic lymphocytic leukemia (CLL). All six CLL patients studied formed B-cell colonies. The number of colonies was significantly less in patients than controls (66 ± 18 versus 127 ± 8). CLL colonies were shown to be monoclonal and appeared to reflect the circulating malignant B-cell clone in our patient group, while the six controls studied formed polyclonal B-cell colonies. Wright-Giemsa staining showed typical plasma cells to have developed in the controls but not in the patients. Cells from CLL patients retained a more lymphoid appearance. It is believed that investigations with this B-cell assay will provide the means for further in vitro evaluation of malignant B-cell proliferation in other lymphoproliferative disorders.",
author = "Perri, {R. T.} and Kay, {Neil Elliot}",
year = "1982",
language = "English (US)",
volume = "59",
pages = "247--249",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "2",

}

TY - JOUR

T1 - Monoclonal CLL B-cells may be induced to grow in an in vitro B-cell colony assay system

AU - Perri, R. T.

AU - Kay, Neil Elliot

PY - 1982

Y1 - 1982

N2 - A simple reproducible in vitro B-cell colony assay system was used to evaluate B-cell growth in controls and patients with chronic lymphocytic leukemia (CLL). All six CLL patients studied formed B-cell colonies. The number of colonies was significantly less in patients than controls (66 ± 18 versus 127 ± 8). CLL colonies were shown to be monoclonal and appeared to reflect the circulating malignant B-cell clone in our patient group, while the six controls studied formed polyclonal B-cell colonies. Wright-Giemsa staining showed typical plasma cells to have developed in the controls but not in the patients. Cells from CLL patients retained a more lymphoid appearance. It is believed that investigations with this B-cell assay will provide the means for further in vitro evaluation of malignant B-cell proliferation in other lymphoproliferative disorders.

AB - A simple reproducible in vitro B-cell colony assay system was used to evaluate B-cell growth in controls and patients with chronic lymphocytic leukemia (CLL). All six CLL patients studied formed B-cell colonies. The number of colonies was significantly less in patients than controls (66 ± 18 versus 127 ± 8). CLL colonies were shown to be monoclonal and appeared to reflect the circulating malignant B-cell clone in our patient group, while the six controls studied formed polyclonal B-cell colonies. Wright-Giemsa staining showed typical plasma cells to have developed in the controls but not in the patients. Cells from CLL patients retained a more lymphoid appearance. It is believed that investigations with this B-cell assay will provide the means for further in vitro evaluation of malignant B-cell proliferation in other lymphoproliferative disorders.

UR - http://www.scopus.com/inward/record.url?scp=0020080558&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020080558&partnerID=8YFLogxK

M3 - Article

C2 - 6976808

AN - SCOPUS:0020080558

VL - 59

SP - 247

EP - 249

JO - Blood

JF - Blood

SN - 0006-4971

IS - 2

ER -