Monoclonal CLL B-cells may be induced to grow in an in vitro B-cell colony assay system

R. T. Perri, N. E. Kay

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A simple reproducible in vitro B-cell colony assay system was used to evaluate B-cell growth in controls and patients with chronic lymphocytic leukemia (CLL). All six CLL patients studied formed B-cell colonies. The number of colonies was significantly less in patients than controls (66 ± 18 versus 127 ± 8). CLL colonies were shown to be monoclonal and appeared to reflect the circulating malignant B-cell clone in our patient group, while the six controls studied formed polyclonal B-cell colonies. Wright-Giemsa staining showed typical plasma cells to have developed in the controls but not in the patients. Cells from CLL patients retained a more lymphoid appearance. It is believed that investigations with this B-cell assay will provide the means for further in vitro evaluation of malignant B-cell proliferation in other lymphoproliferative disorders.

Original languageEnglish (US)
Pages (from-to)247-249
Number of pages3
JournalBlood
Volume59
Issue number2
StatePublished - Jan 1 1982

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ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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