Abstract
A simple reproducible in vitro B-cell colony assay system was used to evaluate B-cell growth in controls and patients with chronic lymphocytic leukemia (CLL). All six CLL patients studied formed B-cell colonies. The number of colonies was significantly less in patients than controls (66 ± 18 versus 127 ± 8). CLL colonies were shown to be monoclonal and appeared to reflect the circulating malignant B-cell clone in our patient group, while the six controls studied formed polyclonal B-cell colonies. Wright-Giemsa staining showed typical plasma cells to have developed in the controls but not in the patients. Cells from CLL patients retained a more lymphoid appearance. It is believed that investigations with this B-cell assay will provide the means for further in vitro evaluation of malignant B-cell proliferation in other lymphoproliferative disorders.
Original language | English (US) |
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Pages (from-to) | 247-249 |
Number of pages | 3 |
Journal | Blood |
Volume | 59 |
Issue number | 2 |
DOIs | |
State | Published - 1982 |
ASJC Scopus subject areas
- Biochemistry
- Immunology
- Hematology
- Cell Biology