Monoclonal antibody analysis of mononuclear cells in myopathies. V: Identification and quantitation of T8+ cytotoxic and T8+ suppressor cells

Kiichi Arahata, Andrew G. Engel

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

In polymyositis (PM) and inclusion body myositis (IBM), but not in dermatomyositis there is evidence of cell‐mediated cytotoxicity: T8+ cells accompanied by macrophages focally surround, invade, and destroy nonnecrotic muscle fibers. However, the T8 marker appears on both cytotoxic (Tc) and suppressor (Ts) cells. The Leu‐15 marker appears on Ts but not on Tc cells, but it also appears on macrophages and on some killer/natural killer cells. To obviate this problem, the T8, Leu‐15, and Leu‐7 markers were demonstrated by sequential paired immunofluorescence in single cryostat sections. Using this approach, we reliably differentiated for the first time between Tc and Ts cells in tissue sections. Six cell phenotypes were identified: T8+Leu‐15 Leu‐7 Tc cells, T8+Leu‐15+Leu‐7 Ts cells, three types of Leu‐7+ killer/natural killer cells, and T8Leu‐15+Leu‐7 macrophages. Muscle specimens from 5 patients with PM and 5 with IBM were studied. In each case, 6 nonnecrotic muscle fibers focally surrounded and invaded by mononuclear cells were selected randomly. A total of 2,022 mononuclear cells were analyzed, 870 from patients with PM and 1,152 from those with IBM. When counts of the identified cell phenotypes in individual patients were pooled, there were four times as many T8+Leu‐15Leu‐7 Ts cells as T8+Leu‐15+Leu‐7 Ts cells in either PM or IBM samples. However, when the relative frequencies of the Tc and Ts cells were examined in individual patients, the Tc cells tended to become more abundant, and the Ts cells correspondingly less abundant, with the duration of symptoms. These data indicate that the T8+ Tc cell is the predominant phenotype in cell‐mediated cytotoxicity in PM and IBM.

Original languageEnglish (US)
Pages (from-to)493-499
Number of pages7
JournalAnnals of neurology
Volume23
Issue number5
DOIs
StatePublished - May 1988

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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