Abstract
Abstract: Eleven unique monoclonal IgG antibodies were raised against rabbit brain acetylcholinesterase (AChE, EC 3.1.1.7), purified to electrophoretic homogeneity by a two‐step procedure involving immunoaffinity chromatography. The apparent dissociation constants of these antibodies for rabbit AChE ranged from about 10 nM to more than 100 nM (assuming one binding site per catalytic subunit). Species cross‐reactivity was investigated with crude brain extracts from rabbit, rat, mouse cat, guinea pig, and human. One antibody bound rabbit AChE exclusively; most bound AChE from three or four species; two bound enzyme from all species tested. Identical, moderate affinity for rat and mouse brain AChE was displayed by two antibodies; two others were able to distinguish between these similar antigens. Nine of the antibodies had lowered affinity for AChE in the presence of 1 M NaCl, but two were salt resistant. Analysis of mutual interferences in AChE binding suggested that certain of the antibodies were competing for nearby epitopes on the AChE surface. One antibody was a potent AChE inhibitor (IC50= 10−8M), blocking up to 90% of the enzyme activity. Most of the antibodies were less able to bind the readily soluble AChE of detergentfree brain extracts than the AChE which required detergent for solubilization. The extreme case, an antibody that was unable to recognize nearly half of the “soluble” AChE, was suspected of lacking affinity for the hydrophilic enzyme form.
Original language | English (US) |
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Pages (from-to) | 284-292 |
Number of pages | 9 |
Journal | Journal of neurochemistry |
Volume | 45 |
Issue number | 1 |
DOIs | |
State | Published - Jul 1985 |
Keywords
- Acetylcholinesterase, hydrophilic and hydrophobic forms
- Acetylcholinesterase, immunoehemistry
- Acetylcholinesterase, mammalian brain
- Monoclonal antibodies, neural acetylcholinesterase
- Monoclonal antibodies, selective inhibition of acetylcholinesterase
ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience