Monoclonal antibodies to purified cortical Lewy bodies recognize the mid-size neurofilament subunit

James E. Galvin, Virginia M Y Lee, Minami Baba, David M A Mann, Dennis W Dickson, Hisako Yamaguchi, M. Luise Schmidt, Takeshi Iwatsubo, John Q. Trojanowski

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Lewy bodies (LBs) are filamentous intraneuronal inclusions that are hallmark lesions of Parkinson's disease, and LBs have been shown, by immunohistochemistry, to contain cytoskeletal as well as other cellular proteins. Similar LBs also occur in the cortical neurons of a subset of patients with Alzheimer's disease (AD), and cortical LBs are the predominant or sole lesions in the brains of patients with an AD-like dementia known as diffuse Lewy-body disease (DLBD). To gain insight into the biochemical composition of LBs, we generated monoclonal antibodies (mAbs) to LBs purified from the brains of patients with DLBD. Here, we describe three of these new mAbs (LB48, LB202, and LB204) that stained LBs by immunohistochemistry and recognized the medium molecular mass neurofilament (NF) protein in western blots. These results support the hypothesis that NF subunits are integral components of LBs. Continued efforts to clarify the composition of LBs are likely to lead to novel strategies for the antemortem diagnosis of LB disorders as well as to insight into the role LBs play in the degeneration of affected neurons in these disorders.

Original languageEnglish (US)
Pages (from-to)595-603
Number of pages9
JournalAnnals of Neurology
Volume42
Issue number4
DOIs
StatePublished - Oct 1997
Externally publishedYes

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Lewy Bodies
Intermediate Filaments
Monoclonal Antibodies
Lewy Body Disease
Alzheimer Disease
Immunohistochemistry
Neurofilament Proteins
Nerve Degeneration
Mass Media
Brain
Parkinson Disease
Dementia
Western Blotting

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Galvin, J. E., Lee, V. M. Y., Baba, M., Mann, D. M. A., Dickson, D. W., Yamaguchi, H., ... Trojanowski, J. Q. (1997). Monoclonal antibodies to purified cortical Lewy bodies recognize the mid-size neurofilament subunit. Annals of Neurology, 42(4), 595-603. https://doi.org/10.1002/ana.410420410

Monoclonal antibodies to purified cortical Lewy bodies recognize the mid-size neurofilament subunit. / Galvin, James E.; Lee, Virginia M Y; Baba, Minami; Mann, David M A; Dickson, Dennis W; Yamaguchi, Hisako; Schmidt, M. Luise; Iwatsubo, Takeshi; Trojanowski, John Q.

In: Annals of Neurology, Vol. 42, No. 4, 10.1997, p. 595-603.

Research output: Contribution to journalArticle

Galvin, JE, Lee, VMY, Baba, M, Mann, DMA, Dickson, DW, Yamaguchi, H, Schmidt, ML, Iwatsubo, T & Trojanowski, JQ 1997, 'Monoclonal antibodies to purified cortical Lewy bodies recognize the mid-size neurofilament subunit', Annals of Neurology, vol. 42, no. 4, pp. 595-603. https://doi.org/10.1002/ana.410420410
Galvin, James E. ; Lee, Virginia M Y ; Baba, Minami ; Mann, David M A ; Dickson, Dennis W ; Yamaguchi, Hisako ; Schmidt, M. Luise ; Iwatsubo, Takeshi ; Trojanowski, John Q. / Monoclonal antibodies to purified cortical Lewy bodies recognize the mid-size neurofilament subunit. In: Annals of Neurology. 1997 ; Vol. 42, No. 4. pp. 595-603.
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