TY - JOUR
T1 - Monoallelic and biallelic deletions of 13q14.3 in chronic lymphocytic leukemia FISH vs miRNA RT-qPCR detection
AU - Smonskey, Matthew T.
AU - Block, Anne Marie W.
AU - Deeb, George
AU - Chanan-Khan, Asher A.
AU - Bernstein, Zale P.
AU - Miller, Kena C.
AU - Wallace, Paul K.
AU - Starostik, Petr
PY - 2012/4
Y1 - 2012/4
N2 - Deletion of 13q14.3 (del(13q)) is the most common cytogenetic abnormality in chronic lymphocytic leukemia (CLL) and implies a favorable prognosis. We explored the feasibility of detecting del(13q) by real-time quantitative polymerase chain reaction (PCR) for miR-15a and miR-16-1, whose loci are located in the deleted region. We analyzed 23 cases of B-CLL with monoallelic (10 cases) or biallelic del(13q) (5 cases) and used trisomy 12-positive CLL samples (n = 8) as control samples. As expected, miR-15a was expressed at significantly lower levels in monoallelic del(13qx1) samples compared with trisomy 12 control samples ( P = .001). Biallelic del(13q) (del(13qx2)) samples showed further reduction of miR-15a levels compared with monoallelic del(13q) (del (13qx1)) ( P = .009). In contrast, miR-16-1 expression levels were generally much lower and variable, with the highest levels detected in del(13qx1). Analyzed retrospectively, miR-15a levels differ among the del(13q) groups. However, only del(13qx2) miR-15a levels are reduced enough to determine the allelic status of an individual sample prospectively by real-time quantitative PCR. Copyright
AB - Deletion of 13q14.3 (del(13q)) is the most common cytogenetic abnormality in chronic lymphocytic leukemia (CLL) and implies a favorable prognosis. We explored the feasibility of detecting del(13q) by real-time quantitative polymerase chain reaction (PCR) for miR-15a and miR-16-1, whose loci are located in the deleted region. We analyzed 23 cases of B-CLL with monoallelic (10 cases) or biallelic del(13q) (5 cases) and used trisomy 12-positive CLL samples (n = 8) as control samples. As expected, miR-15a was expressed at significantly lower levels in monoallelic del(13qx1) samples compared with trisomy 12 control samples ( P = .001). Biallelic del(13q) (del(13qx2)) samples showed further reduction of miR-15a levels compared with monoallelic del(13q) (del (13qx1)) ( P = .009). In contrast, miR-16-1 expression levels were generally much lower and variable, with the highest levels detected in del(13qx1). Analyzed retrospectively, miR-15a levels differ among the del(13q) groups. However, only del(13qx2) miR-15a levels are reduced enough to determine the allelic status of an individual sample prospectively by real-time quantitative PCR. Copyright
KW - Chronic lymphocytic leukemia
KW - Del(13q)
KW - Hsa-miR-15a
KW - Hsa-miR-16-1
KW - MicroRNA
KW - Molecular diagnostics
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UR - http://www.scopus.com/inward/citedby.url?scp=84860618864&partnerID=8YFLogxK
U2 - 10.1309/AJCPP31FSSRQTTAQ
DO - 10.1309/AJCPP31FSSRQTTAQ
M3 - Article
C2 - 22431542
AN - SCOPUS:84860618864
SN - 0002-9173
VL - 137
SP - 641
EP - 646
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 4
ER -