TY - JOUR
T1 - Molecularly cleavable bioinks facilitate high-performance digital light processing-based bioprinting of functional volumetric soft tissues
AU - Wang, Mian
AU - Li, Wanlu
AU - Hao, Jin
AU - Gonzales, Arthur
AU - Zhao, Zhibo
AU - Flores, Regina Sanchez
AU - Kuang, Xiao
AU - Mu, Xuan
AU - Ching, Terry
AU - Tang, Guosheng
AU - Luo, Zeyu
AU - Garciamendez-Mijares, Carlos Ezio
AU - Sahoo, Jugal Kishore
AU - Wells, Michael F.
AU - Niu, Gengle
AU - Agrawal, Prajwal
AU - Quiñones-Hinojosa, Alfredo
AU - Eggan, Kevin
AU - Zhang, Yu Shrike
N1 - Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Digital light processing bioprinting favors biofabrication of tissues with improved structural complexity. However, soft-tissue fabrication with this method remains a challenge to balance the physical performances of the bioinks for high-fidelity bioprinting and suitable microenvironments for the encapsulated cells to thrive. Here, we propose a molecular cleavage approach, where hyaluronic acid methacrylate (HAMA) is mixed with gelatin methacryloyl to achieve high-performance bioprinting, followed by selectively enzymatic digestion of HAMA, resulting in tissue-matching mechanical properties without losing the structural complexity and fidelity. Our method allows cellular morphological and functional improvements across multiple bioprinted tissue types featuring a wide range of mechanical stiffness, from the muscles to the brain, the softest organ of the human body. This platform endows us to biofabricate mechanically precisely tunable constructs to meet the biological function requirements of target tissues, potentially paving the way for broad applications in tissue and tissue model engineering.
AB - Digital light processing bioprinting favors biofabrication of tissues with improved structural complexity. However, soft-tissue fabrication with this method remains a challenge to balance the physical performances of the bioinks for high-fidelity bioprinting and suitable microenvironments for the encapsulated cells to thrive. Here, we propose a molecular cleavage approach, where hyaluronic acid methacrylate (HAMA) is mixed with gelatin methacryloyl to achieve high-performance bioprinting, followed by selectively enzymatic digestion of HAMA, resulting in tissue-matching mechanical properties without losing the structural complexity and fidelity. Our method allows cellular morphological and functional improvements across multiple bioprinted tissue types featuring a wide range of mechanical stiffness, from the muscles to the brain, the softest organ of the human body. This platform endows us to biofabricate mechanically precisely tunable constructs to meet the biological function requirements of target tissues, potentially paving the way for broad applications in tissue and tissue model engineering.
UR - http://www.scopus.com/inward/record.url?scp=85131708584&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85131708584&partnerID=8YFLogxK
U2 - 10.1038/s41467-022-31002-2
DO - 10.1038/s41467-022-31002-2
M3 - Article
C2 - 35680907
AN - SCOPUS:85131708584
SN - 2041-1723
VL - 13
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 3317
ER -