Molecular structure of tail tendon fibers in TIEG1 knockout mice using synchrotron diffraction technology

Laurie Gumez, Sabine F. Bensamoun, Jean Doucet, Oualid Haddad, John R. Hawse, Malayannan Subramaniam, Thomas C. Spelsberg, Chantai Pichon

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Thepurpose of this study was to characterize the effect ot TIEG1 on the molecular structure of collagen within tail tendon fibers using 3-moold female C57BL/6 wild-type (WT) and TIEG1 KO mice. Synchrotron X-ray .microdiffraction experiments were carried out on single tendon fibers extracted, from the WT and TIEGl KO dorsal tail tendon. The fibers were scanned in the radial direction, and X-ray patterns were obtained. From, these patterns, the meridional direction was analyzed through X-ray intensity profile. In addition, collagen content was investigated using hydroxyproline assays, and qualitative real-time PCR experiments were performed on RNA isolated from fibroblasts to examine specific gene expression changes. The results showed different X-ray diffraction patterns between WT and TIEG1 KO tendon fibers, indicating a disorganization of the collagen structure for the TIEG1 KO compared with WT mice. Furthermore, the analyses of the X-ray intensity profiles exhibited a higher (23 Å) period of collagen for the TIEG1 KO compared with the WT mice. The results of the hydroxyproline assays revealed a significant decrease in the TIEG1 KO compared with WT mice, leading to a decrease in the total amount of collagen present within the TIEG1 KO tendons. Moreover, qualitative real-time PCR results showed differences in the expression profiles of specific genes known to play important roles in tendon fiber development. These data further elucidate the role of TIEG1 on tendon structure and could explain the previous defects in the structure-function relationship found for TIEG1 KO tendon fibers.

Original languageEnglish (US)
Pages (from-to)1706-1710
Number of pages5
JournalJournal of applied physiology
Volume108
Issue number6
DOIs
StatePublished - Jun 2010

Keywords

  • Collagen organization
  • Hydroxyproline
  • Qualitative real-time polymerase chain
  • Reaction
  • X-ray

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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    Gumez, L., Bensamoun, S. F., Doucet, J., Haddad, O., Hawse, J. R., Subramaniam, M., Spelsberg, T. C., & Pichon, C. (2010). Molecular structure of tail tendon fibers in TIEG1 knockout mice using synchrotron diffraction technology. Journal of applied physiology, 108(6), 1706-1710. https://doi.org/10.1152/japplphysiol.00356.2010