Molecular specificity of a monoclonal anti-I(k) alloantibody identifying a highly conserved determinant on mouse I-A, I-E and human DR antigens

M. Pierres, W. P. Lafuse, M. Dosseto, C. Devaux, D. Z. Birnbaum, C. S. David

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have investigated, using serological and biochemical assays, the specificity of an A.TH anti-A.TL-derived monoclonal antibody (mAb), designated 40.B, directed at a highly conserved antigenic determinant expressed on the majority of murine and human MHC class II antigens. In the mouse, mAb 40.B defines a new specificity expressed on the Ia products of the H-2 haplotypes k, d, b, v, r, p, u, j and w3. Analysis of its reactivity with H-2 recombinant strains and the results of the competitive binding inhibition of 125I-labeled mAb 40.B to B10.BR cells with I-A(k) and I-E(k) specific mAb suggested recognition of a shared Ia determinant expressed on both I-A(k) and I-E(k) molecules. This has been confirmed by sequential immunoprecipitation studies which demonstrated the specificity of mAb 40.B for the A(k)(β)A(k)(α), A(b)(β)A(b)(α), A(d)(β)A(d)α), E(k)(β)E(k)(α), E(s)(β)E(k)(β) and E(d)(β)E(d)(α) dimers. In humans this mAb bound to an immunoprecipitated HLA-D/DR molecules expressed on lymphoblastoid cell lines carrying the MT1 and MT2 supertypic specificities. The possible implications of these findings with regard to an evolutionary model of MHC class II antigens are discussed.

Original languageEnglish (US)
Pages (from-to)208-220
Number of pages13
JournalTissue Antigens
Volume20
Issue number3
StatePublished - 1982
Externally publishedYes

Fingerprint

Isoantibodies
Monoclonal Antibodies
Antigens
HLA-D Antigens
Competitive Binding
Histocompatibility Antigens Class II
HLA-DR Antigens
Immunoprecipitation
Haplotypes
Epitopes
Cell Line

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Pierres, M., Lafuse, W. P., Dosseto, M., Devaux, C., Birnbaum, D. Z., & David, C. S. (1982). Molecular specificity of a monoclonal anti-I(k) alloantibody identifying a highly conserved determinant on mouse I-A, I-E and human DR antigens. Tissue Antigens, 20(3), 208-220.

Molecular specificity of a monoclonal anti-I(k) alloantibody identifying a highly conserved determinant on mouse I-A, I-E and human DR antigens. / Pierres, M.; Lafuse, W. P.; Dosseto, M.; Devaux, C.; Birnbaum, D. Z.; David, C. S.

In: Tissue Antigens, Vol. 20, No. 3, 1982, p. 208-220.

Research output: Contribution to journalArticle

Pierres, M, Lafuse, WP, Dosseto, M, Devaux, C, Birnbaum, DZ & David, CS 1982, 'Molecular specificity of a monoclonal anti-I(k) alloantibody identifying a highly conserved determinant on mouse I-A, I-E and human DR antigens', Tissue Antigens, vol. 20, no. 3, pp. 208-220.
Pierres, M. ; Lafuse, W. P. ; Dosseto, M. ; Devaux, C. ; Birnbaum, D. Z. ; David, C. S. / Molecular specificity of a monoclonal anti-I(k) alloantibody identifying a highly conserved determinant on mouse I-A, I-E and human DR antigens. In: Tissue Antigens. 1982 ; Vol. 20, No. 3. pp. 208-220.
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