Molecular markers of senescence in fibroblast-like cultures

Vincent J. Cristofalo, Robert J. Pignolo

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

The loss of replicative capacity in vitro of normal human diploid fibroblasts is a model for studying molecular changes that accompany both regulated growth control and cellular senescence. We describe the molecular phenotype of senescent fibroblasts in terms of markers that are altered with proliferative decline. We describe these markers by analyzing pathways and associated mechanisms related to the responsiveness of proliferatively competent and senescent cells to growth signals including changes in the extracellular environment, growth factors, growth factor receptors, secondary messengers, cell-cycle progression, transcription factors, and the fidelity of DNA synthesis. There is an abundance of molecular markers for senescence in culture at every level of information transfer. Although it seems clear that some alterations in gene expression with senescence are the result of specific changes in upstream events, more global dysregulation of coordinated growth control point to as yet undefined mechanisms.

Original languageEnglish (US)
Pages (from-to)111-123
Number of pages13
JournalExperimental Gerontology
Volume31
Issue number1-2
DOIs
StatePublished - Jan 1 1996

Keywords

  • aging
  • cell proliferation
  • cellular senescence
  • growth control
  • signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Cell Biology

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