Molecular Hematopathology

Rong He, Jennifer L. Oliveira, James D. Hoyer, David S. Viswanatha

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Molecular and cytogenetic analysis is a critical component of hematopathology practice. Correct diagnostic classification, prognosis and therapeutic decisions are often predicated on the presence or absence of tumor-specific genetic abnormalities. Understanding the wide range of abnormalities associated with hematologic neoplasia and interpreting a large number of molecular diagnostic techniques can pose challenges for hematopathologists. From chromosome translocations to complex RNA or DNA mutation patterns, the application of molecular diagnosis has greatly enriched our definitions of lymphoid and myeloid neoplasms. The incessant pace of new technological advances such as next-generation sequencing, as well as medical and scientific knowledge accumulation, requires the continuous updating of our knowledge to make judicious decisions regarding cost-effective yet comprehensive approaches to molecularly characterize hematologic cancers. This chapter encompasses an overview of the test design considerations, molecular diagnostic techniques, and rational application of these tests to non-Hodgkin lymphoma and myeloid malignancies in modern hematopathology practice. The field of benign hematology is also greatly expanding with the broader use of molecular analysis. Disorders of hemoglobins, erythrocytosis, and hemolysis have underlying constitutional genetic abnormalities enabling the diagnostic application of molecular testing in an increasingly sophisticated manner. These advances will also be summarized in this chapter.

Original languageEnglish (US)
Title of host publicationHematopathology
Subtitle of host publicationA Volume in the Series: Foundations in Diagnostic Pathology
PublisherElsevier Inc.
Pages712-760.e18
ISBN (Electronic)9780323512312
ISBN (Print)9780323479134
DOIs
StatePublished - Jan 1 2018

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Keywords

  • Cytogenetics
  • FISH
  • Minimal residual disease
  • Molecular basis of red cell diseases
  • Molecular diagnosis of lymphoma and myeloid neoplasms
  • Molecular laboratory techniques
  • Next-generation sequencing
  • PCR
  • Real-time PCR
  • Sequencing
  • Translocations

ASJC Scopus subject areas

  • Medicine(all)

Cite this

He, R., Oliveira, J. L., Hoyer, J. D., & Viswanatha, D. S. (2018). Molecular Hematopathology. In Hematopathology: A Volume in the Series: Foundations in Diagnostic Pathology (pp. 712-760.e18). Elsevier Inc.. https://doi.org/10.1016/B978-0-323-47913-4.00024-0