MOLECULAR, ENZYMATIC AND ULRASTRUCURE CHARACTERIZATION OF THE PYRENOID OF THE SCALY GREEN MONAD MICROMONAS SQUAMATA

Jeffrey L Salisbury, Gary L. Floyd

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Pyrenoid material of micromonas squamata Manton & Parke was obtained free of cell and subcellular particle conamination by differential centrifugation of brei from osmoically lysed cells. The isolated pyrenoid particles were characterized by transmission and scanning electron microscopy. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of pyrenoid extracts revealed a compled polypeptide composition with major components of 12, 54 and 66 kilodalton mol wt. Whole pyrenoids possessed the enzymatic properties of ribulose diphosphate carboxylase and fixed carbon dioxide with specific activity 10 times greaer than that of a pyrenoid‐free high speed supenaant fracion of cell brei. Energy dispersive X‐ray microanalysis revealed he presence of copper in masses of cryo‐impacted pyrenoid material. Ultrastrucural cytochemistry was employed o determine he chemical nature of the reserve carbohydrate shell. Also, the pyrenoid of the intact cell was characterized by transmission electron microscopy.

Original languageEnglish (US)
Pages (from-to)362-368
Number of pages7
JournalJournal of Phycology
Volume14
Issue number3
DOIs
StatePublished - 1978
Externally publishedYes

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Squamata
transmission electron microscopy
carbohydrate
electrokinesis
gel
carbon dioxide
scanning electron microscopy
sodium
cells
shell
sulfate
copper
ribulose
cytochemistry
centrifugation
polyacrylamide gel electrophoresis
energy
polypeptides
conservation areas
carbohydrates

Keywords

  • glutathione reductase
  • green algae
  • Micromonas
  • prasinophyceae
  • pyrenoid
  • RuDP carboxylase

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

MOLECULAR, ENZYMATIC AND ULRASTRUCURE CHARACTERIZATION OF THE PYRENOID OF THE SCALY GREEN MONAD MICROMONAS SQUAMATA. / Salisbury, Jeffrey L; Floyd, Gary L.

In: Journal of Phycology, Vol. 14, No. 3, 1978, p. 362-368.

Research output: Contribution to journalArticle

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