Diagnostic moléculaire du virus de l'immunodéficience humaine acquise (VIH) sur les pools de plasmas de donneurs de sang au centre régional de transfusion sanguine de Ouagadougou (CRST-O), Burkina Faso

Translated title of the contribution: Molecular diagnosis of acquired human immunodeficiency virus (HIV) in pooled plasma from blood donors at the regional blood transfusion center in ouagadougou, Burkina Faso

Bolni Nagalo, C. Bisseye, M. Sanou, Y. K. Nebié, A. Kiba, K. Kienou, J. D. Zongo, J. Simporé

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Study Objectives. The aim of this pilot study was to investigate the use of viral genome diagnosis of HIV-1 infection in blood donors in the regional blood transfusion center in Ouagadougou, Burkina Faso. Methodology. This prospective study was carried out from August to December 2009 at the regional blood transfusion center in Ouagadougou (RBTC-O). Detection of HIV-1 was performed by RT-PCR on pooled plasma and individual samples from blood donors. Samples were selected based on reactivity with fourth generation ELISA. Results. ELISA assays on 20 plasma pools demonstrated 10 negative samples. 8 positive and 2 undeterminable. All positive and negative ELISA tests were confirmed by RT-PCR. Findings of RT-PCR on individual samples confirmed those obtained on pooled plasma samples. For the two undeterminable pools. RT-PCR identified one as negative and the other as positive. Individual RT-PCR testing of donations contained in positive and negative pooled plasma samples confirmed negative or positive findings. Conclusions. Because of the high cost of RT-PCR, we recommend use first on minipools or individual samples from blood donors with questionable HIV-1 status to confirm status quickly and minimize loss of blood bags.

Original languageFrench
Pages (from-to)137-141
Number of pages5
JournalMedecine Tropicale
Volume71
Issue number2
StatePublished - Apr 1 2011
Externally publishedYes

Fingerprint

Burkina Faso
Blood Donors
Blood Transfusion
HIV
Polymerase Chain Reaction
HIV-1
Enzyme-Linked Immunosorbent Assay
Viral Genome
Virus Diseases
Prospective Studies
Costs and Cost Analysis

Keywords

  • Blood donor
  • Blood safety
  • Burkina Faso
  • HIV-1
  • RT-PCR

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Diagnostic moléculaire du virus de l'immunodéficience humaine acquise (VIH) sur les pools de plasmas de donneurs de sang au centre régional de transfusion sanguine de Ouagadougou (CRST-O), Burkina Faso. / Nagalo, Bolni; Bisseye, C.; Sanou, M.; Nebié, Y. K.; Kiba, A.; Kienou, K.; Zongo, J. D.; Simporé, J.

In: Medecine Tropicale, Vol. 71, No. 2, 01.04.2011, p. 137-141.

Research output: Contribution to journalArticle

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abstract = "Study Objectives. The aim of this pilot study was to investigate the use of viral genome diagnosis of HIV-1 infection in blood donors in the regional blood transfusion center in Ouagadougou, Burkina Faso. Methodology. This prospective study was carried out from August to December 2009 at the regional blood transfusion center in Ouagadougou (RBTC-O). Detection of HIV-1 was performed by RT-PCR on pooled plasma and individual samples from blood donors. Samples were selected based on reactivity with fourth generation ELISA. Results. ELISA assays on 20 plasma pools demonstrated 10 negative samples. 8 positive and 2 undeterminable. All positive and negative ELISA tests were confirmed by RT-PCR. Findings of RT-PCR on individual samples confirmed those obtained on pooled plasma samples. For the two undeterminable pools. RT-PCR identified one as negative and the other as positive. Individual RT-PCR testing of donations contained in positive and negative pooled plasma samples confirmed negative or positive findings. Conclusions. Because of the high cost of RT-PCR, we recommend use first on minipools or individual samples from blood donors with questionable HIV-1 status to confirm status quickly and minimize loss of blood bags.",
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