Molecular determinants of calpain-dependent cleavage of junctophilin-2 protein in cardiomyocytes

Ang Guo; Duane Hall; Caimei Zhang; Tianqing Peng; Jordan D. Miller; William Kutschke; Chad E. Grueter; Frances L. Johnson; Richard Z. Lin; Long Sheng Song

doi:10.1074/jbc.M115.652396

Molecular determinants of calpain-dependent cleavage of junctophilin-2 protein in cardiomyocytes

Ang Guo, Duane Hall, Caimei Zhang, Tianqing Peng, Jordan D. Miller, William Kutschke, Chad E. Grueter, Frances L. Johnson, Richard Z. Lin, Long Sheng Song

Cardiovascular Surgery

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Background: Down-regulation of junctophilin-2 is associated with a variety of cardiac diseases. Results: Junctophilin-2 is cleaved by calpain at multiple sites, resulting in dysfunctional junctophilin-2 truncations. Conclusion: Calpain-mediated proteolysis contributes to posttranslational down-regulation of junctophilin-2. Significance: These data reveal, for the first time, the detailed molecular determinants responsible for calpain proteolysis of junctophilin-2.

Original language	English (US)
Pages (from-to)	17946-17955
Number of pages	10
Journal	Journal of Biological Chemistry
Volume	290
Issue number	29
DOIs	https://doi.org/10.1074/jbc.M115.652396
State	Published - Jul 17 2015

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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title = "Molecular determinants of calpain-dependent cleavage of junctophilin-2 protein in cardiomyocytes",

abstract = "Background: Down-regulation of junctophilin-2 is associated with a variety of cardiac diseases. Results: Junctophilin-2 is cleaved by calpain at multiple sites, resulting in dysfunctional junctophilin-2 truncations. Conclusion: Calpain-mediated proteolysis contributes to posttranslational down-regulation of junctophilin-2. Significance: These data reveal, for the first time, the detailed molecular determinants responsible for calpain proteolysis of junctophilin-2.",

author = "Ang Guo and Duane Hall and Caimei Zhang and Tianqing Peng and Miller, {Jordan D.} and William Kutschke and Grueter, {Chad E.} and Johnson, {Frances L.} and Lin, {Richard Z.} and Song, {Long Sheng}",

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T1 - Molecular determinants of calpain-dependent cleavage of junctophilin-2 protein in cardiomyocytes

AU - Guo, Ang

AU - Hall, Duane

AU - Zhang, Caimei

AU - Peng, Tianqing

AU - Miller, Jordan D.

AU - Kutschke, William

AU - Grueter, Chad E.

AU - Johnson, Frances L.

AU - Lin, Richard Z.

AU - Song, Long Sheng

PY - 2015/7/17

Y1 - 2015/7/17

N2 - Background: Down-regulation of junctophilin-2 is associated with a variety of cardiac diseases. Results: Junctophilin-2 is cleaved by calpain at multiple sites, resulting in dysfunctional junctophilin-2 truncations. Conclusion: Calpain-mediated proteolysis contributes to posttranslational down-regulation of junctophilin-2. Significance: These data reveal, for the first time, the detailed molecular determinants responsible for calpain proteolysis of junctophilin-2.

AB - Background: Down-regulation of junctophilin-2 is associated with a variety of cardiac diseases. Results: Junctophilin-2 is cleaved by calpain at multiple sites, resulting in dysfunctional junctophilin-2 truncations. Conclusion: Calpain-mediated proteolysis contributes to posttranslational down-regulation of junctophilin-2. Significance: These data reveal, for the first time, the detailed molecular determinants responsible for calpain proteolysis of junctophilin-2.

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Molecular determinants of calpain-dependent cleavage of junctophilin-2 protein in cardiomyocytes

Abstract

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