Molecular determinants of calpain-dependent cleavage of junctophilin-2 protein in cardiomyocytes

Ang Guo, Duane Hall, Caimei Zhang, Tianqing Peng, Jordan D. Miller, William Kutschke, Chad E. Grueter, Frances L. Johnson, Richard Z. Lin, Long Sheng Song

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Down-regulation of junctophilin-2 is associated with a variety of cardiac diseases. Results: Junctophilin-2 is cleaved by calpain at multiple sites, resulting in dysfunctional junctophilin-2 truncations. Conclusion: Calpain-mediated proteolysis contributes to posttranslational down-regulation of junctophilin-2. Significance: These data reveal, for the first time, the detailed molecular determinants responsible for calpain proteolysis of junctophilin-2.

Original languageEnglish (US)
Pages (from-to)17946-17955
Number of pages10
JournalJournal of Biological Chemistry
Volume290
Issue number29
DOIs
StatePublished - Jul 17 2015

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Calpain
Cardiac Myocytes
Proteolysis
Proteins
Down-Regulation
Heart Diseases
junctophilin

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Guo, A., Hall, D., Zhang, C., Peng, T., Miller, J. D., Kutschke, W., ... Song, L. S. (2015). Molecular determinants of calpain-dependent cleavage of junctophilin-2 protein in cardiomyocytes. Journal of Biological Chemistry, 290(29), 17946-17955. https://doi.org/10.1074/jbc.M115.652396

Molecular determinants of calpain-dependent cleavage of junctophilin-2 protein in cardiomyocytes. / Guo, Ang; Hall, Duane; Zhang, Caimei; Peng, Tianqing; Miller, Jordan D.; Kutschke, William; Grueter, Chad E.; Johnson, Frances L.; Lin, Richard Z.; Song, Long Sheng.

In: Journal of Biological Chemistry, Vol. 290, No. 29, 17.07.2015, p. 17946-17955.

Research output: Contribution to journalArticle

Guo, A, Hall, D, Zhang, C, Peng, T, Miller, JD, Kutschke, W, Grueter, CE, Johnson, FL, Lin, RZ & Song, LS 2015, 'Molecular determinants of calpain-dependent cleavage of junctophilin-2 protein in cardiomyocytes', Journal of Biological Chemistry, vol. 290, no. 29, pp. 17946-17955. https://doi.org/10.1074/jbc.M115.652396
Guo, Ang ; Hall, Duane ; Zhang, Caimei ; Peng, Tianqing ; Miller, Jordan D. ; Kutschke, William ; Grueter, Chad E. ; Johnson, Frances L. ; Lin, Richard Z. ; Song, Long Sheng. / Molecular determinants of calpain-dependent cleavage of junctophilin-2 protein in cardiomyocytes. In: Journal of Biological Chemistry. 2015 ; Vol. 290, No. 29. pp. 17946-17955.
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