TY - JOUR
T1 - Molecular cloning of the human eosinophil peroxidase. Evidence for the existence of a peroxidase multigene family
AU - Ten, R. M.
AU - Pease, L. R.
AU - McKean, D. J.
AU - Bell, M. P.
AU - Gleich, G. J.
PY - 1989
Y1 - 1989
N2 - Human eosinophil peroxidase (EPO) was purified from eosinophil granules derived from the peripheral blood of patients with eosinophilia. The molecular mass of the H and L subunits was determined by gel filtration to be 57,000 and 11,000 daltons, respectively. The partial amino acid sequences of both subunits were used to construct oligonucleotides for the screening of several cDNA libraries, including one derived from human-induced umbilical cord mononuclear cells. A cDNA clone was isolated corresponding to EPO. The nucleotide sequence revealed an open reading frame of 2,106 bp, corresponding to a prosequence, L chain, and H chain, in this order. Comparison of the EPO nucleotide sequence with other peroxidases, such as myeloperoxidase, suggests the existence of a multigene family.
AB - Human eosinophil peroxidase (EPO) was purified from eosinophil granules derived from the peripheral blood of patients with eosinophilia. The molecular mass of the H and L subunits was determined by gel filtration to be 57,000 and 11,000 daltons, respectively. The partial amino acid sequences of both subunits were used to construct oligonucleotides for the screening of several cDNA libraries, including one derived from human-induced umbilical cord mononuclear cells. A cDNA clone was isolated corresponding to EPO. The nucleotide sequence revealed an open reading frame of 2,106 bp, corresponding to a prosequence, L chain, and H chain, in this order. Comparison of the EPO nucleotide sequence with other peroxidases, such as myeloperoxidase, suggests the existence of a multigene family.
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U2 - 10.1084/jem.169.5.1757
DO - 10.1084/jem.169.5.1757
M3 - Article
C2 - 2541222
AN - SCOPUS:0024508002
SN - 0022-1007
VL - 169
SP - 1757
EP - 1769
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 5
ER -