Molecular cloning and nucleotide sequence of Streptomyces griseus trypsin gene

Jee Cheon Kim, Seung Hee Cha, Seong Tae Jeong, SangKon Oh, Si Myung Byun

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Streptomyces griseus trypsin (E.C.3.4.21.4) is one of the major extracellular proteinase, which is secreted by S. griseus. The gene encoding S. griseus trypsin was isolated from a S. griseus genomic library by using a synthetic oligonucleotide probe. Fragments containing the gene for S. griseus trypsin were characterized by hybridization and demonstration of proteolytic activity in S. lividans. Deduced amino acid sequence from the nucleotide sequence suggests that S. griseus trypsin is produced as a precursor, consisting of three portions; an amino-terminal pre sequence (32 amino acid residues), a pro sequence (4 residues), and the mature trypsin. The S. griseus trypsin consists of 223 amino acids with a computed molecular weight of 23,112. The existence of proline at the pro and mature junction suggests that the processing of S. griseus trypsin is non-autocatalytic.

Original languageEnglish (US)
Pages (from-to)707-713
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume181
Issue number2
DOIs
StatePublished - Dec 16 1991
Externally publishedYes

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Streptomyces griseus
Cloning
Molecular Cloning
Trypsin
Nucleotides
Genes
Amino Acids
Amino Acid Sequence
Gene encoding
Genomic Library
Oligonucleotide Probes
Proline
Peptide Hydrolases
Demonstrations
Molecular weight
Molecular Weight
Processing

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Molecular cloning and nucleotide sequence of Streptomyces griseus trypsin gene. / Kim, Jee Cheon; Cha, Seung Hee; Jeong, Seong Tae; Oh, SangKon; Byun, Si Myung.

In: Biochemical and Biophysical Research Communications, Vol. 181, No. 2, 16.12.1991, p. 707-713.

Research output: Contribution to journalArticle

Kim, Jee Cheon ; Cha, Seung Hee ; Jeong, Seong Tae ; Oh, SangKon ; Byun, Si Myung. / Molecular cloning and nucleotide sequence of Streptomyces griseus trypsin gene. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 181, No. 2. pp. 707-713.
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