Molecular Cloning and Functional Expression of the Human Gallbladder Cholecystokinin A Receptor

Charles D. Ulrich, Irene Ferber, Eileen Holicky, Elizabeth Hadac, Gary Buell, Laurence J. Miller

Research output: Contribution to journalArticle

91 Scopus citations

Abstract

Through binding to cholecystokinin (CCK) A receptors, CCK is an important physiologic regulator of both gallbladder contraction and pancreatic enzyme secretion. In this work, we have used a combination of hybridization screening of a cDNA library and polymerase chain reaction to clone a 2.1 kb cDNA which encodes the human gallbladder CCKA receptor. Nucleotide sequence analysis revealed an open reading frame encoding a 428 amino acid protein, with seven putative transmembrane domains and a high degree of homology with the rat CCKA receptor. COS cells transfected with this cDNA clone bound CCK-8 and L-364,718 with high affinities appropriate for the CCKA receptor, and exhibited a transient increase in intracellular calcium in response to CCK. This should provide an important resource for the analysis of the role of this receptor in human physiology and pathophysiology.

Original languageEnglish (US)
Pages (from-to)204-211
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume193
Issue number1
DOIs
StatePublished - May 28 1993

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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