Molecular characterization of human osteoblast-derived extracellular vesicle mRNA using next-generation sequencing

Jess Morhayim, Jeroen van de Peppel, Amel Dudakovic, Hideki Chiba, Andre J van Wijnen, Johannes P. van Leeuwen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Extracellular vesicles (EVs) are membrane-bound intercellular communication vehicles that transport proteins, lipids and nucleic acids with regulatory capacity between cells. RNA profiling using microarrays and sequencing technologies has revolutionized the discovery of EV-RNA content, which is crucial to understand the molecular mechanism of EV function. Recent studies have indicated that EVs are enriched with specific RNAs compared to the originating cells suggestive of an active sorting mechanism. Here, we present the comparative transcriptome analysis of human osteoblasts and their corresponding EVs using next-generation sequencing. We demonstrate that osteoblast-EVs are specifically depleted of cellular mRNAs that encode proteins involved in basic cellular activities, such as cytoskeletal functions, cell survival and apoptosis. In contrast, EVs are significantly enriched with 254 mRNAs that are associated with protein translation and RNA processing. Moreover, mRNAs enriched in EVs encode proteins important for communication with the neighboring cells, in particular with osteoclasts, adipocytes and hematopoietic stem cells. These findings provide the foundation for understanding the molecular mechanism and function of EV-mediated interactions between osteoblasts and the surrounding bone microenvironment.

Original languageEnglish (US)
Pages (from-to)1133-1141
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1864
Issue number7
DOIs
StatePublished - Jul 1 2017

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Osteoblasts
Messenger RNA
RNA
Extracellular Vesicles
Protein Biosynthesis
Gene Expression Profiling
Osteoclasts
Hematopoietic Stem Cells
Adipocytes
Nucleic Acids
Cell Survival
Carrier Proteins
Proteins
Communication
Apoptosis
Technology
Lipids
Bone and Bones
Membranes

Keywords

  • Extracellular mRNA
  • Extracellular vesicles
  • Intercellular communication
  • Next-generation sequencing
  • Osteoblasts

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Molecular characterization of human osteoblast-derived extracellular vesicle mRNA using next-generation sequencing. / Morhayim, Jess; van de Peppel, Jeroen; Dudakovic, Amel; Chiba, Hideki; van Wijnen, Andre J; van Leeuwen, Johannes P.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1864, No. 7, 01.07.2017, p. 1133-1141.

Research output: Contribution to journalArticle

Morhayim, Jess ; van de Peppel, Jeroen ; Dudakovic, Amel ; Chiba, Hideki ; van Wijnen, Andre J ; van Leeuwen, Johannes P. / Molecular characterization of human osteoblast-derived extracellular vesicle mRNA using next-generation sequencing. In: Biochimica et Biophysica Acta - Molecular Cell Research. 2017 ; Vol. 1864, No. 7. pp. 1133-1141.
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