Molecular characterization of a swelling-induced chloride conductance regulatory protein, plCln

Grigory B. Krapivinsky, Michael John Ackerman, Eric A. Gordon, Lyubov D. Krapivinsky, David E. Clapham

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

Cells maintain control of their volume by the passage of KCl and water across their membranes, but the regulatory proteins are unknown. Expression in Xenopus oocytes of a novel protein, plCln, activated a chloride conductance. We have cloned analogs of plCln from rat heart and Xenopus ovary, plCln was identified as an abundant soluble cytosolic protein (∼-40 kd) that does not immunolocalize with the plasma membrane, plein was found in epithelial and cardiac cells, brain, and Xenopus oocytes, forming complexes with soluble actin and other cytosolic proteins. Monoclonal antibodies recognizing plCln blocked activation of a native hypotonicity-induced chloride conductance (ICl.swell) in Xenopus oocytes, suggesting that plCln may link act inbound cytoskeletal elements to an unidentified volume-sensitive chloride channel. The high degree of sequence conservation and widespread expression of plCln suggest that it is an important element in cellular volume regulation.

Original languageEnglish (US)
Pages (from-to)439-448
Number of pages10
JournalCell
Volume76
Issue number3
StatePublished - 1994

Fingerprint

Xenopus
Swelling
Chlorides
Oocytes
Proteins
Chloride Channels
Cell membranes
Rats
Actins
Ovary
Conservation
Brain
Membrane Proteins
Epithelial Cells
Chemical activation
Monoclonal Antibodies
Cell Membrane
Membranes
Water

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Krapivinsky, G. B., Ackerman, M. J., Gordon, E. A., Krapivinsky, L. D., & Clapham, D. E. (1994). Molecular characterization of a swelling-induced chloride conductance regulatory protein, plCln Cell, 76(3), 439-448.

Molecular characterization of a swelling-induced chloride conductance regulatory protein, plCln . / Krapivinsky, Grigory B.; Ackerman, Michael John; Gordon, Eric A.; Krapivinsky, Lyubov D.; Clapham, David E.

In: Cell, Vol. 76, No. 3, 1994, p. 439-448.

Research output: Contribution to journalArticle

Krapivinsky, GB, Ackerman, MJ, Gordon, EA, Krapivinsky, LD & Clapham, DE 1994, 'Molecular characterization of a swelling-induced chloride conductance regulatory protein, plCln ', Cell, vol. 76, no. 3, pp. 439-448.
Krapivinsky GB, Ackerman MJ, Gordon EA, Krapivinsky LD, Clapham DE. Molecular characterization of a swelling-induced chloride conductance regulatory protein, plCln Cell. 1994;76(3):439-448.
Krapivinsky, Grigory B. ; Ackerman, Michael John ; Gordon, Eric A. ; Krapivinsky, Lyubov D. ; Clapham, David E. / Molecular characterization of a swelling-induced chloride conductance regulatory protein, plCln In: Cell. 1994 ; Vol. 76, No. 3. pp. 439-448.
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