Molecular characterization and functional expression of opioid receptor-likei receptor

Ya Lan Wu, Lu Pu, Kun Ling, Jian Zhao, Zhi Jie Cheng, Lan Ma, Gang Pei

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The opioid receptor-likei receptor (ORL1), an orphan receptor whose human and murine complementary DNAs, has been characterized recently. ORL1 transcripts are particularly abundant in the central nervous system. We demonstrated that ORL1 expressed in human neuroblastoma SK-N-SH and SH-SY5Y cell lines by radioligand binding assay, reverse transcription polymerase chain reaction (RT-PCR) and Northern analysis in the present study. Stimulation with ORL1 specific agonist, nociceptin/orphanin FQ, increased [35S]GTPλ binding to SK-N-SH cell membranes (EC50 = 14±0.45 nM), and attenuated forskolin-stimulated accumulation of cellular cAMP (EC50 = 0.80 ±.45 nM), indicative that activation of ORL1 activates G proteins and inhibits adenylyl cyclase. Activation of ORL1 receptor was also accessed using CHO:hORL1 cell line by microphysiometer. Treatment of nociceptin/orphanin FQ increased extracellular acidification rate significantly.

Original languageEnglish (US)
Pages (from-to)69-77
Number of pages9
JournalCell Research
Volume7
Issue number1
StatePublished - 1997
Externally publishedYes

Fingerprint

Opioid Receptors
Cell Line
Radioligand Assay
CHO Cells
Colforsin
Guanosine Triphosphate
Neuroblastoma
GTP-Binding Proteins
Adenylyl Cyclases
Reverse Transcription
Central Nervous System
Complementary DNA
Cell Membrane
Polymerase Chain Reaction
nociceptin receptor
nociceptin

Keywords

  • Extracellular acidification
  • Functional expression
  • Nociceptin/orphanin FQ (N/OFQ)
  • Opioid receptor-likei receptor (ORL)
  • SK-NSH cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Wu, Y. L., Pu, L., Ling, K., Zhao, J., Cheng, Z. J., Ma, L., & Pei, G. (1997). Molecular characterization and functional expression of opioid receptor-likei receptor. Cell Research, 7(1), 69-77.

Molecular characterization and functional expression of opioid receptor-likei receptor. / Wu, Ya Lan; Pu, Lu; Ling, Kun; Zhao, Jian; Cheng, Zhi Jie; Ma, Lan; Pei, Gang.

In: Cell Research, Vol. 7, No. 1, 1997, p. 69-77.

Research output: Contribution to journalArticle

Wu, YL, Pu, L, Ling, K, Zhao, J, Cheng, ZJ, Ma, L & Pei, G 1997, 'Molecular characterization and functional expression of opioid receptor-likei receptor', Cell Research, vol. 7, no. 1, pp. 69-77.
Wu, Ya Lan ; Pu, Lu ; Ling, Kun ; Zhao, Jian ; Cheng, Zhi Jie ; Ma, Lan ; Pei, Gang. / Molecular characterization and functional expression of opioid receptor-likei receptor. In: Cell Research. 1997 ; Vol. 7, No. 1. pp. 69-77.
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