Molecular basis of genetic polymorphism in major histocompatibility complex-linked proteasome gene (Lmp-2)

P. Zhou, H. Cao, M. Smart, C. David

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Four genes, closely linked to major histocompatibility complex (MHC) class II genes, have been identified in humans, mice, and rats and are thought to be involved in the generation and transport of endogenous immunogenic peptides for the MHC class I antigen-processing pathway. The Tap-1 and Tap-2 genes presumably encode a heterodimeric protein complex responsible for transporting endogenous immunogenic peptides to the lumen of the endoplasmic reticulum. The Lmp-2 and Lmp-7 gene products are two subunits of the large cytosolic proteasome complex possibly involved in generation of endogenous peptides. To study the genetic polymorphism of the Lmp-2 gene, we used a published cDNA sequence as a consensus sequence and PCR-amplified, cloned, and sequenced the Lmp-2 gene from 12 inbred mouse strains. We found three amino acid variants, LMP-2(d), LMP-2b, and LMP-2(q), which partially correlated with restriction fragment length polymorphism variants identified with Southern blots. Allelic polymorphism of the Lmp-2 gene may be involved in peptide selection, leading to autoimmune disease susceptibility.

Original languageEnglish (US)
Pages (from-to)2681-2684
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number7
StatePublished - 1993

Fingerprint

Genetic Polymorphisms
Proteasome Endopeptidase Complex
Major Histocompatibility Complex
Molecular Biology
Genes
Peptides
MHC Class II Genes
Histocompatibility Antigens Class I
Inbred Strains Mice
Disease Susceptibility
Consensus Sequence
Antigen Presentation
Southern Blotting
Endoplasmic Reticulum
Restriction Fragment Length Polymorphisms
Autoimmune Diseases
Complementary DNA
Amino Acids
Polymerase Chain Reaction
Proteins

Keywords

  • class I molecules
  • peptides
  • processing

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Molecular basis of genetic polymorphism in major histocompatibility complex-linked proteasome gene (Lmp-2). / Zhou, P.; Cao, H.; Smart, M.; David, C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 7, 1993, p. 2681-2684.

Research output: Contribution to journalArticle

@article{6c5ff434bcd64e1f977a2fe969922056,
title = "Molecular basis of genetic polymorphism in major histocompatibility complex-linked proteasome gene (Lmp-2)",
abstract = "Four genes, closely linked to major histocompatibility complex (MHC) class II genes, have been identified in humans, mice, and rats and are thought to be involved in the generation and transport of endogenous immunogenic peptides for the MHC class I antigen-processing pathway. The Tap-1 and Tap-2 genes presumably encode a heterodimeric protein complex responsible for transporting endogenous immunogenic peptides to the lumen of the endoplasmic reticulum. The Lmp-2 and Lmp-7 gene products are two subunits of the large cytosolic proteasome complex possibly involved in generation of endogenous peptides. To study the genetic polymorphism of the Lmp-2 gene, we used a published cDNA sequence as a consensus sequence and PCR-amplified, cloned, and sequenced the Lmp-2 gene from 12 inbred mouse strains. We found three amino acid variants, LMP-2(d), LMP-2b, and LMP-2(q), which partially correlated with restriction fragment length polymorphism variants identified with Southern blots. Allelic polymorphism of the Lmp-2 gene may be involved in peptide selection, leading to autoimmune disease susceptibility.",
keywords = "class I molecules, peptides, processing",
author = "P. Zhou and H. Cao and M. Smart and C. David",
year = "1993",
language = "English (US)",
volume = "90",
pages = "2681--2684",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "7",

}

TY - JOUR

T1 - Molecular basis of genetic polymorphism in major histocompatibility complex-linked proteasome gene (Lmp-2)

AU - Zhou, P.

AU - Cao, H.

AU - Smart, M.

AU - David, C.

PY - 1993

Y1 - 1993

N2 - Four genes, closely linked to major histocompatibility complex (MHC) class II genes, have been identified in humans, mice, and rats and are thought to be involved in the generation and transport of endogenous immunogenic peptides for the MHC class I antigen-processing pathway. The Tap-1 and Tap-2 genes presumably encode a heterodimeric protein complex responsible for transporting endogenous immunogenic peptides to the lumen of the endoplasmic reticulum. The Lmp-2 and Lmp-7 gene products are two subunits of the large cytosolic proteasome complex possibly involved in generation of endogenous peptides. To study the genetic polymorphism of the Lmp-2 gene, we used a published cDNA sequence as a consensus sequence and PCR-amplified, cloned, and sequenced the Lmp-2 gene from 12 inbred mouse strains. We found three amino acid variants, LMP-2(d), LMP-2b, and LMP-2(q), which partially correlated with restriction fragment length polymorphism variants identified with Southern blots. Allelic polymorphism of the Lmp-2 gene may be involved in peptide selection, leading to autoimmune disease susceptibility.

AB - Four genes, closely linked to major histocompatibility complex (MHC) class II genes, have been identified in humans, mice, and rats and are thought to be involved in the generation and transport of endogenous immunogenic peptides for the MHC class I antigen-processing pathway. The Tap-1 and Tap-2 genes presumably encode a heterodimeric protein complex responsible for transporting endogenous immunogenic peptides to the lumen of the endoplasmic reticulum. The Lmp-2 and Lmp-7 gene products are two subunits of the large cytosolic proteasome complex possibly involved in generation of endogenous peptides. To study the genetic polymorphism of the Lmp-2 gene, we used a published cDNA sequence as a consensus sequence and PCR-amplified, cloned, and sequenced the Lmp-2 gene from 12 inbred mouse strains. We found three amino acid variants, LMP-2(d), LMP-2b, and LMP-2(q), which partially correlated with restriction fragment length polymorphism variants identified with Southern blots. Allelic polymorphism of the Lmp-2 gene may be involved in peptide selection, leading to autoimmune disease susceptibility.

KW - class I molecules

KW - peptides

KW - processing

UR - http://www.scopus.com/inward/record.url?scp=0027398939&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027398939&partnerID=8YFLogxK

M3 - Article

VL - 90

SP - 2681

EP - 2684

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 7

ER -