Molecular basis for binding and subtype selectivity of 1,4-benzodiazepine antagonist ligands of the cholecystokinin receptor

Erin E. Cawston, Polo C H Lam, Kaleeckal G. Harikumar, Maoqing Dong, Alicja M. Ball, Mary Lou Augustine, Eyup Akgün, Philip S. Portoghese, Andrew Orry, Ruben Abagyan, Patrick M. Sexton, Laurence J Miller

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Allosteric binding pockets in peptide-binding G protein-coupled receptors create opportunities for the development of small molecule drugs with substantial benefits over orthosteric ligands. To gain insights into molecular determinants for this pocket within type 1 and 2 cholecystokinin receptors (CCK1R and CCK2R), we prepared a series of receptor constructs in which six distinct residues in TM2, -3, -6, and -7 were reversed. Two novel iodinated CCK1R- and CCK2R-selective 1,4-benzodiazepine antagonists, differing only in stereochemistry at C3, were used. When all six residues within CCK1R were mutated to corresponding CCK2R residues, benzodiazepine selectivity was reversed, yet peptide binding selectivity was unaffected. Detailed analysis, including observations of gain of function, demonstrated that residues 6.51, 6.52, and 7.39 were most important for binding the CCK1R-selective ligand, whereas residues 2.61 and 7.39 were most important for binding CCK2Rselective ligand, although the effect of substitution of residue 2.61 was likely indirect. Ligand-guided homology modeling was applied to wild type receptors and those reversing benzodiazepine binding selectivity. The models had high predictive power in enriching known receptor-selective ligands from related decoys, indicating a high degree of precision in pocket definition. The benzodiazepines docked in similar poses in both receptors, with C3 urea substituents pointing upward, whereas different stereochemistry at C3 directed the C5 phenyl rings and N1 methyl groups into opposite orientations. The geometry of the binding pockets and specific interactions predicted for ligand docking in these models provide a molecular framework for understanding ligand selectivity at these receptor subtypes. Furthermore, the strong predictive power of these models suggests their usefulness in the discovery of lead compounds and in drug development programs.

Original languageEnglish (US)
Pages (from-to)18618-18635
Number of pages18
JournalJournal of Biological Chemistry
Volume287
Issue number22
DOIs
StatePublished - May 25 2012

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Cholecystokinin Receptors
Ligands
Benzodiazepines
Stereochemistry
Cholecystokinin B Receptor
Lead compounds
Peptides
G-Protein-Coupled Receptors
Bz-423
Pharmaceutical Preparations
Urea
Carrier Proteins
Substitution reactions
Molecules
Geometry

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Molecular basis for binding and subtype selectivity of 1,4-benzodiazepine antagonist ligands of the cholecystokinin receptor. / Cawston, Erin E.; Lam, Polo C H; Harikumar, Kaleeckal G.; Dong, Maoqing; Ball, Alicja M.; Augustine, Mary Lou; Akgün, Eyup; Portoghese, Philip S.; Orry, Andrew; Abagyan, Ruben; Sexton, Patrick M.; Miller, Laurence J.

In: Journal of Biological Chemistry, Vol. 287, No. 22, 25.05.2012, p. 18618-18635.

Research output: Contribution to journalArticle

Cawston, EE, Lam, PCH, Harikumar, KG, Dong, M, Ball, AM, Augustine, ML, Akgün, E, Portoghese, PS, Orry, A, Abagyan, R, Sexton, PM & Miller, LJ 2012, 'Molecular basis for binding and subtype selectivity of 1,4-benzodiazepine antagonist ligands of the cholecystokinin receptor', Journal of Biological Chemistry, vol. 287, no. 22, pp. 18618-18635. https://doi.org/10.1074/jbc.M111.335646
Cawston, Erin E. ; Lam, Polo C H ; Harikumar, Kaleeckal G. ; Dong, Maoqing ; Ball, Alicja M. ; Augustine, Mary Lou ; Akgün, Eyup ; Portoghese, Philip S. ; Orry, Andrew ; Abagyan, Ruben ; Sexton, Patrick M. ; Miller, Laurence J. / Molecular basis for binding and subtype selectivity of 1,4-benzodiazepine antagonist ligands of the cholecystokinin receptor. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 22. pp. 18618-18635.
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