Abstract
Molecular genetic approaches for colorectal cancer screening using stool samples have the potential to be very specific, sensitive and cost effective. K-ras oncogene mutation, observed in about 50% of the colorectal tumours, serves as an ideal target for such an approach. Several studies have shown that K-ras gene mutations present in the tumours can be detected from the stool of patients with colorectal cancer. The challenge for clinical application, however, is to develop a simple method for robust PCR amplification and a reliable assay for K-ras gene mutation detection in the stool samples.
Original language | English (US) |
---|---|
Pages (from-to) | 213-217 |
Number of pages | 5 |
Journal | European Journal of Gastroenterology and Hepatology |
Volume | 10 |
Issue number | 3 |
DOIs | |
State | Published - 1998 |
Keywords
- Aberrant crypt foci
- Colorectal cancer screening
- Early diagnosis
- K-ras mutation
- Mis-match ligation assay
- PCR
- Stool DNA purification
ASJC Scopus subject areas
- Hepatology
- Gastroenterology