Molecular and serological examination of the relationship of human herpesvirus 8 to multiple myeloma: orf 26 sequences in bone marrow stroma are not restricted to myeloma patients and other regions of the genome are not detected

John F. Tisdale, Alexander Keith Stewart, Bruce Dickstein, Richard F. Little, Ian Dubé, D. Cappe, Cynthia E. Dunbar, Kevin E. Brown

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Abstract

Human herpesvirus 8 (HHV-8) genomic sequences were recently detected by polymerase chain reaction (PCR) and in situ hybridization in bone marrow stromal cells grown from multiple myeloma (MM) patients, but not in cells from control subjects (Rettig et al, Science 276:1851, 1997). We sought to confirm these observations in our own group of MM patients (n = 30). DNA was extracted from adherent stromal cells grown under varying conditions and assayed for HHV-8 sequence using PCR to amplify the orf 26 (KS330) sequence (Chang et al, Science 266:1865, 1997), as initially reported. Samples from human control subjects (n = 25) were concurrently extracted and analyzed. After 30 cycles of amplification, we did not detect any positive samples. In a more sensitive nested PCR, samples from 18 of 30 (60%) MM patients were positive, at about the limit of detection, but orf 26 sequence was also amplified from 11 of 25 (44%) human control samples. However, PCR amplification from other regions of the viral genome (orf 72 and orf 75) was uniformly negative for all MM and control samples, despite equivalent sensitivity. Additionally, all sera from MM patients were negative for HHV-8 IgG by immunofluorescence. Our data do not support a role of HHV-8 in the etiology of MM but may suggest the presence of a related (KS330-containing) virus in MM patients and in some control subjects.

Original languageEnglish (US)
Pages (from-to)2681-2687
Number of pages7
JournalBlood
Volume92
Issue number8
StatePublished - Oct 15 1998
Externally publishedYes

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Contagious Ecthyma
Human Herpesvirus 8
Multiple Myeloma
Polymerase chain reaction
Bone
Genes
Bone Marrow
Genome
Polymerase Chain Reaction
Amplification
Cells
Viruses
Viral Genome
Immunoglobulin G
Stromal Cells
Mesenchymal Stromal Cells
In Situ Hybridization
Fluorescent Antibody Technique
Limit of Detection
DNA

ASJC Scopus subject areas

  • Hematology

Cite this

Molecular and serological examination of the relationship of human herpesvirus 8 to multiple myeloma : orf 26 sequences in bone marrow stroma are not restricted to myeloma patients and other regions of the genome are not detected. / Tisdale, John F.; Stewart, Alexander Keith; Dickstein, Bruce; Little, Richard F.; Dubé, Ian; Cappe, D.; Dunbar, Cynthia E.; Brown, Kevin E.

In: Blood, Vol. 92, No. 8, 15.10.1998, p. 2681-2687.

Research output: Contribution to journalArticle

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abstract = "Human herpesvirus 8 (HHV-8) genomic sequences were recently detected by polymerase chain reaction (PCR) and in situ hybridization in bone marrow stromal cells grown from multiple myeloma (MM) patients, but not in cells from control subjects (Rettig et al, Science 276:1851, 1997). We sought to confirm these observations in our own group of MM patients (n = 30). DNA was extracted from adherent stromal cells grown under varying conditions and assayed for HHV-8 sequence using PCR to amplify the orf 26 (KS330) sequence (Chang et al, Science 266:1865, 1997), as initially reported. Samples from human control subjects (n = 25) were concurrently extracted and analyzed. After 30 cycles of amplification, we did not detect any positive samples. In a more sensitive nested PCR, samples from 18 of 30 (60{\%}) MM patients were positive, at about the limit of detection, but orf 26 sequence was also amplified from 11 of 25 (44{\%}) human control samples. However, PCR amplification from other regions of the viral genome (orf 72 and orf 75) was uniformly negative for all MM and control samples, despite equivalent sensitivity. Additionally, all sera from MM patients were negative for HHV-8 IgG by immunofluorescence. Our data do not support a role of HHV-8 in the etiology of MM but may suggest the presence of a related (KS330-containing) virus in MM patients and in some control subjects.",
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AU - Stewart, Alexander Keith

AU - Dickstein, Bruce

AU - Little, Richard F.

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