MOLECULAR ANALYSIS OF TWO RECOMBINANT MOUSE STRAINS WITH CROSSOVERS IN THE Eα RECOMBINATION HOT SPOT

W. P. Lafuse, S. T. Lee, C. S. David

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Recombination in the Eα gene of the mouse major histocompatibility complex has been found to occur only in mice derived from crosses between strains with the k and p haplotypes. In the present paper the crossover sites of six additional strains were examined by RFLP analysis. These recombinant strains were derived from crosses between strains (B10.RPD1, B10.RPD2) with crossovers in the Eα gene and B10.M (H‐2f). Four have crossover sites between the Eα gene and the S region. One recombinant (B10.RFD7) also crossed over within the Eα gene and a second (B10.RPF1) crossed over within a segment located to the left of the Eα gene. This study suggests that the DNA sequence responsible for recombination in the Eα gene is also present in the B10.RPD1 and B10.RPD2 recombinant strains.

Original languageEnglish (US)
Pages (from-to)169-176
Number of pages8
JournalInternational Journal of Immunogenetics
Volume17
Issue number3
DOIs
StatePublished - Jun 1990

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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