MOLECULAR ANALYSIS OF TWO RECOMBINANT MOUSE STRAINS WITH CROSSOVERS IN THE Eα RECOMBINATION HOT SPOT

W. P. Lafuse; S. T. Lee; C. S. David

doi:10.1111/j.1744-313X.1990.tb00869.x

MOLECULAR ANALYSIS OF TWO RECOMBINANT MOUSE STRAINS WITH CROSSOVERS IN THE E_α RECOMBINATION HOT SPOT

W. P. Lafuse, S. T. Lee, C. S. David

Immunology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Recombination in the E_α gene of the mouse major histocompatibility complex has been found to occur only in mice derived from crosses between strains with the k and p haplotypes. In the present paper the crossover sites of six additional strains were examined by RFLP analysis. These recombinant strains were derived from crosses between strains (B10.RPD1, B10.RPD2) with crossovers in the E_α gene and B10.M (H‐2^f). Four have crossover sites between the E_α gene and the S region. One recombinant (B10.RFD7) also crossed over within the E_α gene and a second (B10.RPF1) crossed over within a segment located to the left of the E_α gene. This study suggests that the DNA sequence responsible for recombination in the E_α gene is also present in the B10.RPD1 and B10.RPD2 recombinant strains.

Original language	English (US)
Pages (from-to)	169-176
Number of pages	8
Journal	International Journal of Immunogenetics
Volume	17
Issue number	3
DOIs	https://doi.org/10.1111/j.1744-313X.1990.tb00869.x
State	Published - Jun 1990

ASJC Scopus subject areas

Immunology
Molecular Biology
Genetics
Genetics(clinical)

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abstract = "Recombination in the Eα gene of the mouse major histocompatibility complex has been found to occur only in mice derived from crosses between strains with the k and p haplotypes. In the present paper the crossover sites of six additional strains were examined by RFLP analysis. These recombinant strains were derived from crosses between strains (B10.RPD1, B10.RPD2) with crossovers in the Eα gene and B10.M (H‐2f). Four have crossover sites between the Eα gene and the S region. One recombinant (B10.RFD7) also crossed over within the Eα gene and a second (B10.RPF1) crossed over within a segment located to the left of the Eα gene. This study suggests that the DNA sequence responsible for recombination in the Eα gene is also present in the B10.RPD1 and B10.RPD2 recombinant strains.",

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MOLECULAR ANALYSIS OF TWO RECOMBINANT MOUSE STRAINS WITH CROSSOVERS IN THE E_α RECOMBINATION HOT SPOT

Abstract

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