Abstract
The monitoring of gastric lymphomas is often hampered by the inherently limited sampling provided by small endoscopic biopsy specimens. To investigate the feasibility of using gastric washing fluid for monitoring patients with known gastric lymphoma and for diagnosing gastric involvement in patients with extranodal nongastric lymphoma, we collected 49 gastric washings from 39 patients (29 patients with gastric lymphoma and 10 patients with nongastric extranodal lymphoma). Collection was done at the time of follow-up biopsy and when no endoscopic abnormalities were found. DNA was extracted from the washing fluid and analyzed for clonal IgH gene rearrangement by Southern blotting (J6 probe) and/or polymerase chain reaction (PCR) (using VH-FR3 and JH primers). Forty-one of 49 samples (84%) yielded sufficient DNA for molecular analysis, Sixteen of 41 analyzable gastric washing samples (39%) failed Southern blot analysis due to degraded or insufficient DNA. Concordance between the results of Southern blot analysis of the washing and histology of the simultaneous biopsy specimen was found in 20 (80%) of the remaining 25 samples. The IgH PCR result was concordant with biopsy histology in 33 out of 41 washing samples (80%). The overall concordance between molecular clonality studies of washings (Southern blotting and/or PCR) and biopsy histology was 83% (34 of 41). Of the 7 (18%) discrepant specimens, 2 were diagnosed histologically as lymphoma, but the simultaneous washings were negative by molecular studies. Five biopsy specimens were histologically benign, but the corresponding washings demonstrated clonal IgH gene rearrangement (3 cases by PCR and 2 cases by Southern blotting). This study demonstrates the diagnostic utility of molecular clonality analysis of gastric washings.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 582-586 |
| Number of pages | 5 |
| Journal | Human Pathology |
| Volume | 35 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2004 |
| Externally published | Yes |
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Keywords
- Clonality
- Gastric lavage
- Gastric lymphoma
- IgH gene rearrangement
- Mucosa-associated lymphoid tissue
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Molecular analysis of gastric washings in the diagnosis and monitoring of gastric lymphomas. / Chen, Beiyun; Colleoni, Gisele W B; Salazar, Paulo A.; Lal, Priti; Gerdes, Hans; Filippa, Daniel A.
In: Human Pathology, Vol. 35, No. 5, 05.2004, p. 582-586.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular analysis of gastric washings in the diagnosis and monitoring of gastric lymphomas
AU - Chen, Beiyun
AU - Colleoni, Gisele W B
AU - Salazar, Paulo A.
AU - Lal, Priti
AU - Gerdes, Hans
AU - Filippa, Daniel A.
PY - 2004/5
Y1 - 2004/5
N2 - The monitoring of gastric lymphomas is often hampered by the inherently limited sampling provided by small endoscopic biopsy specimens. To investigate the feasibility of using gastric washing fluid for monitoring patients with known gastric lymphoma and for diagnosing gastric involvement in patients with extranodal nongastric lymphoma, we collected 49 gastric washings from 39 patients (29 patients with gastric lymphoma and 10 patients with nongastric extranodal lymphoma). Collection was done at the time of follow-up biopsy and when no endoscopic abnormalities were found. DNA was extracted from the washing fluid and analyzed for clonal IgH gene rearrangement by Southern blotting (J6 probe) and/or polymerase chain reaction (PCR) (using VH-FR3 and JH primers). Forty-one of 49 samples (84%) yielded sufficient DNA for molecular analysis, Sixteen of 41 analyzable gastric washing samples (39%) failed Southern blot analysis due to degraded or insufficient DNA. Concordance between the results of Southern blot analysis of the washing and histology of the simultaneous biopsy specimen was found in 20 (80%) of the remaining 25 samples. The IgH PCR result was concordant with biopsy histology in 33 out of 41 washing samples (80%). The overall concordance between molecular clonality studies of washings (Southern blotting and/or PCR) and biopsy histology was 83% (34 of 41). Of the 7 (18%) discrepant specimens, 2 were diagnosed histologically as lymphoma, but the simultaneous washings were negative by molecular studies. Five biopsy specimens were histologically benign, but the corresponding washings demonstrated clonal IgH gene rearrangement (3 cases by PCR and 2 cases by Southern blotting). This study demonstrates the diagnostic utility of molecular clonality analysis of gastric washings.
AB - The monitoring of gastric lymphomas is often hampered by the inherently limited sampling provided by small endoscopic biopsy specimens. To investigate the feasibility of using gastric washing fluid for monitoring patients with known gastric lymphoma and for diagnosing gastric involvement in patients with extranodal nongastric lymphoma, we collected 49 gastric washings from 39 patients (29 patients with gastric lymphoma and 10 patients with nongastric extranodal lymphoma). Collection was done at the time of follow-up biopsy and when no endoscopic abnormalities were found. DNA was extracted from the washing fluid and analyzed for clonal IgH gene rearrangement by Southern blotting (J6 probe) and/or polymerase chain reaction (PCR) (using VH-FR3 and JH primers). Forty-one of 49 samples (84%) yielded sufficient DNA for molecular analysis, Sixteen of 41 analyzable gastric washing samples (39%) failed Southern blot analysis due to degraded or insufficient DNA. Concordance between the results of Southern blot analysis of the washing and histology of the simultaneous biopsy specimen was found in 20 (80%) of the remaining 25 samples. The IgH PCR result was concordant with biopsy histology in 33 out of 41 washing samples (80%). The overall concordance between molecular clonality studies of washings (Southern blotting and/or PCR) and biopsy histology was 83% (34 of 41). Of the 7 (18%) discrepant specimens, 2 were diagnosed histologically as lymphoma, but the simultaneous washings were negative by molecular studies. Five biopsy specimens were histologically benign, but the corresponding washings demonstrated clonal IgH gene rearrangement (3 cases by PCR and 2 cases by Southern blotting). This study demonstrates the diagnostic utility of molecular clonality analysis of gastric washings.
KW - Clonality
KW - Gastric lavage
KW - Gastric lymphoma
KW - IgH gene rearrangement
KW - Mucosa-associated lymphoid tissue
UR - http://www.scopus.com/inward/record.url?scp=2442701521&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442701521&partnerID=8YFLogxK
U2 - 10.1016/j.humpath.2003.12.008
DO - 10.1016/j.humpath.2003.12.008
M3 - Article
C2 - 15138933
AN - SCOPUS:2442701521
VL - 35
SP - 582
EP - 586
JO - Human Pathology
JF - Human Pathology
SN - 0046-8177
IS - 5
ER -