Modulation of marrow stromal cell function using poly(D,L-lactic acid)- block-poly(ethylene glycol)-monomethyl ether surfaces

Achim Göpferich; Susan J. Peter; Andrea Lucke; Lichun Lu; Antonios G. Mikos

doi:10.1002/(SICI)1097-4636(19990905)46:3<390::AID-JBM12>3.0.CO;2-N

Modulation of marrow stromal cell function using poly(D,L-lactic acid)- block-poly(ethylene glycol)-monomethyl ether surfaces

Achim Göpferich, Susan J. Peter, Andrea Lucke, Lichun Lu, Antonios G. Mikos

Orthopedic Surgery

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

The adhesion of marrow stromal osteoblasts and the adsorption of fetal bovine serum (FBS) proteins to end-capped poly(D,L-lactic acid) 50:50 (PLA50) of molecular weight 17,000 (PLA50₁₇), non-end-capped PLA50 of molecular weight 11,000 (PLA50(11h)), and a diblock copolymer made of poly(ethylene glycol)-monomethyl ether of molecular weight 5,000 and PLA50 of molecular weight 20,000 (Me.PEG5-PLA20) were investigated. Cell attachment and proliferation on both PLA50 polymers were equally good. The block copolymer did not allow the proliferation of cells. However, the attached cells were highly differentiated and metabolically active in contrast to the cells on PLA50. More-over, surface analysis studies using electron spectroscopy revealed that FBS proteins adsorbed well from aqueous solutions to the PLA50 surfaces while they adsorbed substantially less to the block copolymer. These results suggest that Me.PEG-PLA block copolymers may be used to regulate protein adsorption and, therefore, cell adhesion by varying the block composition of the copolymer.

Original language	English (US)
Pages (from-to)	390-398
Number of pages	9
Journal	Journal of Biomedical Materials Research
Volume	46
Issue number	3
DOIs	https://doi.org/10.1002/(SICI)1097-4636(19990905)46:3<390::AID-JBM12>3.0.CO;2-N
State	Published - Sep 5 1999

Keywords

Block copolymer
Cell adhesion
Degradable polymer
Protein adsorption
Stromal cell

ASJC Scopus subject areas

Biomaterials
Biomedical Engineering

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10.1002/(SICI)1097-4636(19990905)46:3<390::AID-JBM12>3.0.CO;2-N

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title = "Modulation of marrow stromal cell function using poly(D,L-lactic acid)- block-poly(ethylene glycol)-monomethyl ether surfaces",

abstract = "The adhesion of marrow stromal osteoblasts and the adsorption of fetal bovine serum (FBS) proteins to end-capped poly(D,L-lactic acid) 50:50 (PLA50) of molecular weight 17,000 (PLA5017), non-end-capped PLA50 of molecular weight 11,000 (PLA50(11h)), and a diblock copolymer made of poly(ethylene glycol)-monomethyl ether of molecular weight 5,000 and PLA50 of molecular weight 20,000 (Me.PEG5-PLA20) were investigated. Cell attachment and proliferation on both PLA50 polymers were equally good. The block copolymer did not allow the proliferation of cells. However, the attached cells were highly differentiated and metabolically active in contrast to the cells on PLA50. More-over, surface analysis studies using electron spectroscopy revealed that FBS proteins adsorbed well from aqueous solutions to the PLA50 surfaces while they adsorbed substantially less to the block copolymer. These results suggest that Me.PEG-PLA block copolymers may be used to regulate protein adsorption and, therefore, cell adhesion by varying the block composition of the copolymer.",

keywords = "Block copolymer, Cell adhesion, Degradable polymer, Protein adsorption, Stromal cell",

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year = "1999",

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doi = "10.1002/(SICI)1097-4636(19990905)46:3<390::AID-JBM12>3.0.CO;2-N",

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AU - Göpferich, Achim

AU - Peter, Susan J.

AU - Lucke, Andrea

AU - Lu, Lichun

AU - Mikos, Antonios G.

PY - 1999/9/5

Y1 - 1999/9/5

N2 - The adhesion of marrow stromal osteoblasts and the adsorption of fetal bovine serum (FBS) proteins to end-capped poly(D,L-lactic acid) 50:50 (PLA50) of molecular weight 17,000 (PLA5017), non-end-capped PLA50 of molecular weight 11,000 (PLA50(11h)), and a diblock copolymer made of poly(ethylene glycol)-monomethyl ether of molecular weight 5,000 and PLA50 of molecular weight 20,000 (Me.PEG5-PLA20) were investigated. Cell attachment and proliferation on both PLA50 polymers were equally good. The block copolymer did not allow the proliferation of cells. However, the attached cells were highly differentiated and metabolically active in contrast to the cells on PLA50. More-over, surface analysis studies using electron spectroscopy revealed that FBS proteins adsorbed well from aqueous solutions to the PLA50 surfaces while they adsorbed substantially less to the block copolymer. These results suggest that Me.PEG-PLA block copolymers may be used to regulate protein adsorption and, therefore, cell adhesion by varying the block composition of the copolymer.

AB - The adhesion of marrow stromal osteoblasts and the adsorption of fetal bovine serum (FBS) proteins to end-capped poly(D,L-lactic acid) 50:50 (PLA50) of molecular weight 17,000 (PLA5017), non-end-capped PLA50 of molecular weight 11,000 (PLA50(11h)), and a diblock copolymer made of poly(ethylene glycol)-monomethyl ether of molecular weight 5,000 and PLA50 of molecular weight 20,000 (Me.PEG5-PLA20) were investigated. Cell attachment and proliferation on both PLA50 polymers were equally good. The block copolymer did not allow the proliferation of cells. However, the attached cells were highly differentiated and metabolically active in contrast to the cells on PLA50. More-over, surface analysis studies using electron spectroscopy revealed that FBS proteins adsorbed well from aqueous solutions to the PLA50 surfaces while they adsorbed substantially less to the block copolymer. These results suggest that Me.PEG-PLA block copolymers may be used to regulate protein adsorption and, therefore, cell adhesion by varying the block composition of the copolymer.

KW - Block copolymer

KW - Cell adhesion

KW - Degradable polymer

KW - Protein adsorption

KW - Stromal cell

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Modulation of marrow stromal cell function using poly(D,L-lactic acid)- block-poly(ethylene glycol)-monomethyl ether surfaces

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Keywords

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