Modulation of lymphohematopoiesis in long-term cultures by gamma interferon: Direct and indirect action on lymphoid and stromal cells

J. M. Gimble, K. Medina, J. Hudson, M. Robinson, P. W. Kincade

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Gamma interferon (IFN-γ) is the product of multiple cell types within the bone marrow microenvironment and has been demonstrated to act as a potent inhibitor of myelopoiesis in vitro and in vivo. The action of this cytokine on lymphohemopoiesis has now been examined on both long-term bone marrow cultures and representative cloned cellular components of the bone marrow microenvironment. In myelopoietic (Dexter) cultures, the half maximal inhibitory concentration of IFN-γ was between 1 and 10 U/mL. In comparable lymphopoietic (Whitlock/Witte) cultures, IFN-γ inhibited the production of B-lineage lymphoid cells with a half maximal effective concentration of less than 1 U/mL. In a clonal assay for pre-B cells, IFN-γ inhibited colony formation with a half maximal concentration of 1 to 5 U/mL. Not all B- lineage lymphoid cells displayed the same sensitivity, however. Growth of the IL-7-dependent B cell line (2E8) in methyl-cellulose assays was unaffected by IFN-γ while the replication of other lymphoid lines was partially or completely inhibited. IFN-γ induced the expression of cell surface proteins (MHC Class I and II) on both B-lineage cells and stromal cells. In cloned stromal cell lines, IFN-γ increased the steady state mRNA levels for the cytokines interleukin-6 (IL-6) and JE, a member of the IL-8 family. These data indicate that IFN-γ acts within the lymphohematopoietic microenvironment through both direct and indirect actions on the hemopoietic and stromal cell populations.

Original languageEnglish (US)
Pages (from-to)224-230
Number of pages7
JournalExperimental Hematology
Volume21
Issue number2
StatePublished - 1993

Keywords

  • IFN-γ
  • Long-term cultures
  • Lymphohematopoiesis
  • Stromal cells

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

Fingerprint Dive into the research topics of 'Modulation of lymphohematopoiesis in long-term cultures by gamma interferon: Direct and indirect action on lymphoid and stromal cells'. Together they form a unique fingerprint.

  • Cite this