Modulation of cyclooxygenase-2 on glycine- and glutamate-induced ion currents in rat periaqueductal gray neurons

Min Chul Shin, Mi Hyeon Jang, Hyun Kyung Chang, Youn Jung Kim, Ee Hwa Kim, Chang Ju Kim

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Cyclooxygenase (COX) is a key enzyme in the conversion of arachidonic acid to prostaglandins. Two isoforms of COX are known: COX-1 and COX-2. In the present study, the modulatory effect of COX-2 on glycine- and glutamate-induced ion currents in periaqueductal gray (PAG) neurons was investigated using the nystatin-perforated patch clamp method. Continuous application of lipopolysaccharides on PAG neurons resulted in increased glycine-induced ion current and decreased glutamate-induced ion current. In contrast, continuous application of celecoxib, selective COX-2 inhibitor, resulted in decreased glycine-induced ion current and increased glutamate-induced ion current. These results demonstrate that COX-2 modulates neuronal activity of PAG, and it can be suggested that COX-2 participates in the regulation of the descending pain control system in the level of PAG neurons.

Original languageEnglish (US)
Pages (from-to)251-256
Number of pages6
JournalBrain Research Bulletin
Volume59
Issue number4
DOIs
StatePublished - Jan 15 2003

Keywords

  • Cyclooxygenase-2
  • Glutamate
  • Glycine
  • Patch clamp
  • Periaqueductal gray neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Modulation of cyclooxygenase-2 on glycine- and glutamate-induced ion currents in rat periaqueductal gray neurons'. Together they form a unique fingerprint.

  • Cite this