Modulation of collagen gene expression by cytokines: Stimulatory effect of transforming growth factor-β1, with divergent effects of epidermal growth factor and tumor necrosis factor-α on collagen type I and collagen type IV

Joseph Peter Grande, Deborah C. Melder, Alan R. Zinsmeister

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Transforming growth factor-β1 (TGF-β1) is well recognized as a patient mediator of both fibrillar (collagen type I) and basement membrane (collagen type IV) production. However, tissue injury is characterized by the concomitant expression of many cytokines and/or growth factors in addition to TGF-β1, and the ultimate extent of extracellular-matrix (ECM) deposition may reflect the interacting effects of TGF-β1 and these other cytokines and/or growth factors. We, therefore, sought to determine whether other cytokines and/or growth factors, known to be produced after tissue injury, one capable either alone or in combination with TGF-β1 of modulating collagen gene expression. Collagen type I and collagen type IV gene expression was assessed in NIH-3T3 cells, a murine fibroblast-like cell line that responds to TGF-β1, with increases in both collagen type I and collagen type IV production. TGF-β1 coordinately induced production of collagen type IV messenger ribonucleic acid (mRNA) to a level 3.8-fold above its baseline value (p < 0.001) and collagen type I mRNA to a level 2.6-fold above its baseline value (p < 0.001). Of the other cytokines and/or growth factors tested, only epidermal growth factor (EGF) had significant effects on collagen mRNA expression. We report the novel observation that EGF significantly induced collagen type IV mRNA (3.0-fold; p < 0.001) but did not alter collagen type I mRNA expression. Platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), and insulin-like growth factor-1 (IGF-1) did not alter the expression of mRNA for collagen type IV or collagen type I. Addition of TGF-β1 to cytokine- and/or growth factor-treated cells increased both collagen type IV and collagen type I mRNA levels. However, collagen type IV mRNA levels were similar in cultures given TGF-β1 alone and cultures given TGF-β1 with other cytokines and/or growth factors; there were no additive, synergistic, or antagonistic effects after coadministration of TGF-β1 and other cytokines and/or growth factors. With regard to collagen type I mRNA expression, all cytokines and/or growth factors tested, with the exception of TNF-α, had no effect on collagen type I mRNA levels in TGF-β1 -treated cultures. Importantly, TNF-α antagonized the stimulatory effect of TGF-β1 on collagen type I mRNA levels. These observations support a dominant role for TGF-β1 in stimulating coordinate expression of collagen type I and collagen type IV mRNA by NIH-3T3 cells; EGF and TNF-α are capable of inducing divergent expression of the genes for these two types of collagen.

Original languageEnglish (US)
Pages (from-to)476-486
Number of pages11
JournalJournal of Laboratory and Clinical Medicine
Volume130
Issue number5
StatePublished - Nov 1997

Fingerprint

Collagen Type IV
Transforming Growth Factors
Collagen Type I
Epidermal Growth Factor
Gene expression
Collagen
Tumor Necrosis Factor-alpha
Modulation
Cytokines
Gene Expression
RNA
Intercellular Signaling Peptides and Proteins
NIH 3T3 Cells
Cells
Fibrillar Collagens
Tissue
Platelet-Derived Growth Factor
Wounds and Injuries
Somatomedins
Fibroblast Growth Factor 2

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

@article{e47174d6f5e344eab462c22ab5d47741,
title = "Modulation of collagen gene expression by cytokines: Stimulatory effect of transforming growth factor-β1, with divergent effects of epidermal growth factor and tumor necrosis factor-α on collagen type I and collagen type IV",
abstract = "Transforming growth factor-β1 (TGF-β1) is well recognized as a patient mediator of both fibrillar (collagen type I) and basement membrane (collagen type IV) production. However, tissue injury is characterized by the concomitant expression of many cytokines and/or growth factors in addition to TGF-β1, and the ultimate extent of extracellular-matrix (ECM) deposition may reflect the interacting effects of TGF-β1 and these other cytokines and/or growth factors. We, therefore, sought to determine whether other cytokines and/or growth factors, known to be produced after tissue injury, one capable either alone or in combination with TGF-β1 of modulating collagen gene expression. Collagen type I and collagen type IV gene expression was assessed in NIH-3T3 cells, a murine fibroblast-like cell line that responds to TGF-β1, with increases in both collagen type I and collagen type IV production. TGF-β1 coordinately induced production of collagen type IV messenger ribonucleic acid (mRNA) to a level 3.8-fold above its baseline value (p < 0.001) and collagen type I mRNA to a level 2.6-fold above its baseline value (p < 0.001). Of the other cytokines and/or growth factors tested, only epidermal growth factor (EGF) had significant effects on collagen mRNA expression. We report the novel observation that EGF significantly induced collagen type IV mRNA (3.0-fold; p < 0.001) but did not alter collagen type I mRNA expression. Platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), and insulin-like growth factor-1 (IGF-1) did not alter the expression of mRNA for collagen type IV or collagen type I. Addition of TGF-β1 to cytokine- and/or growth factor-treated cells increased both collagen type IV and collagen type I mRNA levels. However, collagen type IV mRNA levels were similar in cultures given TGF-β1 alone and cultures given TGF-β1 with other cytokines and/or growth factors; there were no additive, synergistic, or antagonistic effects after coadministration of TGF-β1 and other cytokines and/or growth factors. With regard to collagen type I mRNA expression, all cytokines and/or growth factors tested, with the exception of TNF-α, had no effect on collagen type I mRNA levels in TGF-β1 -treated cultures. Importantly, TNF-α antagonized the stimulatory effect of TGF-β1 on collagen type I mRNA levels. These observations support a dominant role for TGF-β1 in stimulating coordinate expression of collagen type I and collagen type IV mRNA by NIH-3T3 cells; EGF and TNF-α are capable of inducing divergent expression of the genes for these two types of collagen.",
author = "Grande, {Joseph Peter} and Melder, {Deborah C.} and Zinsmeister, {Alan R.}",
year = "1997",
month = "11",
language = "English (US)",
volume = "130",
pages = "476--486",
journal = "Translational Research",
issn = "1931-5244",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - Modulation of collagen gene expression by cytokines

T2 - Stimulatory effect of transforming growth factor-β1, with divergent effects of epidermal growth factor and tumor necrosis factor-α on collagen type I and collagen type IV

AU - Grande, Joseph Peter

AU - Melder, Deborah C.

AU - Zinsmeister, Alan R.

PY - 1997/11

Y1 - 1997/11

N2 - Transforming growth factor-β1 (TGF-β1) is well recognized as a patient mediator of both fibrillar (collagen type I) and basement membrane (collagen type IV) production. However, tissue injury is characterized by the concomitant expression of many cytokines and/or growth factors in addition to TGF-β1, and the ultimate extent of extracellular-matrix (ECM) deposition may reflect the interacting effects of TGF-β1 and these other cytokines and/or growth factors. We, therefore, sought to determine whether other cytokines and/or growth factors, known to be produced after tissue injury, one capable either alone or in combination with TGF-β1 of modulating collagen gene expression. Collagen type I and collagen type IV gene expression was assessed in NIH-3T3 cells, a murine fibroblast-like cell line that responds to TGF-β1, with increases in both collagen type I and collagen type IV production. TGF-β1 coordinately induced production of collagen type IV messenger ribonucleic acid (mRNA) to a level 3.8-fold above its baseline value (p < 0.001) and collagen type I mRNA to a level 2.6-fold above its baseline value (p < 0.001). Of the other cytokines and/or growth factors tested, only epidermal growth factor (EGF) had significant effects on collagen mRNA expression. We report the novel observation that EGF significantly induced collagen type IV mRNA (3.0-fold; p < 0.001) but did not alter collagen type I mRNA expression. Platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), and insulin-like growth factor-1 (IGF-1) did not alter the expression of mRNA for collagen type IV or collagen type I. Addition of TGF-β1 to cytokine- and/or growth factor-treated cells increased both collagen type IV and collagen type I mRNA levels. However, collagen type IV mRNA levels were similar in cultures given TGF-β1 alone and cultures given TGF-β1 with other cytokines and/or growth factors; there were no additive, synergistic, or antagonistic effects after coadministration of TGF-β1 and other cytokines and/or growth factors. With regard to collagen type I mRNA expression, all cytokines and/or growth factors tested, with the exception of TNF-α, had no effect on collagen type I mRNA levels in TGF-β1 -treated cultures. Importantly, TNF-α antagonized the stimulatory effect of TGF-β1 on collagen type I mRNA levels. These observations support a dominant role for TGF-β1 in stimulating coordinate expression of collagen type I and collagen type IV mRNA by NIH-3T3 cells; EGF and TNF-α are capable of inducing divergent expression of the genes for these two types of collagen.

AB - Transforming growth factor-β1 (TGF-β1) is well recognized as a patient mediator of both fibrillar (collagen type I) and basement membrane (collagen type IV) production. However, tissue injury is characterized by the concomitant expression of many cytokines and/or growth factors in addition to TGF-β1, and the ultimate extent of extracellular-matrix (ECM) deposition may reflect the interacting effects of TGF-β1 and these other cytokines and/or growth factors. We, therefore, sought to determine whether other cytokines and/or growth factors, known to be produced after tissue injury, one capable either alone or in combination with TGF-β1 of modulating collagen gene expression. Collagen type I and collagen type IV gene expression was assessed in NIH-3T3 cells, a murine fibroblast-like cell line that responds to TGF-β1, with increases in both collagen type I and collagen type IV production. TGF-β1 coordinately induced production of collagen type IV messenger ribonucleic acid (mRNA) to a level 3.8-fold above its baseline value (p < 0.001) and collagen type I mRNA to a level 2.6-fold above its baseline value (p < 0.001). Of the other cytokines and/or growth factors tested, only epidermal growth factor (EGF) had significant effects on collagen mRNA expression. We report the novel observation that EGF significantly induced collagen type IV mRNA (3.0-fold; p < 0.001) but did not alter collagen type I mRNA expression. Platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), and insulin-like growth factor-1 (IGF-1) did not alter the expression of mRNA for collagen type IV or collagen type I. Addition of TGF-β1 to cytokine- and/or growth factor-treated cells increased both collagen type IV and collagen type I mRNA levels. However, collagen type IV mRNA levels were similar in cultures given TGF-β1 alone and cultures given TGF-β1 with other cytokines and/or growth factors; there were no additive, synergistic, or antagonistic effects after coadministration of TGF-β1 and other cytokines and/or growth factors. With regard to collagen type I mRNA expression, all cytokines and/or growth factors tested, with the exception of TNF-α, had no effect on collagen type I mRNA levels in TGF-β1 -treated cultures. Importantly, TNF-α antagonized the stimulatory effect of TGF-β1 on collagen type I mRNA levels. These observations support a dominant role for TGF-β1 in stimulating coordinate expression of collagen type I and collagen type IV mRNA by NIH-3T3 cells; EGF and TNF-α are capable of inducing divergent expression of the genes for these two types of collagen.

UR - http://www.scopus.com/inward/record.url?scp=0031281979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031281979&partnerID=8YFLogxK

M3 - Article

C2 - 9390635

AN - SCOPUS:0031281979

VL - 130

SP - 476

EP - 486

JO - Translational Research

JF - Translational Research

SN - 1931-5244

IS - 5

ER -